Animal-Free IL-3 蛋白, Human (His)

Animal-Free IL-3 Protein, Human (His) is the recombinant human-derived animal-FreeIL-3 protein, expressed by E. coli, with C-His labeled tag., has molecular weight of ~16 kDa.This product is for cell culture use only.

IL-3蛋白是一种主要由活化的T淋巴细胞、肥大细胞和成骨细胞分泌的细胞因子，在调节造血祖细胞的产生和分化为特定细胞谱系中发挥着至关重要的作用。此外，IL-3 还可刺激成熟嗜碱性粒细胞、嗜酸性粒细胞和单核细胞的功能激活。它还在神经细胞增殖和存活中具有重要功能，并通过防止 NF-κ-B 核转位和激活来抑制破骨细胞分化，从而参与维持骨稳态。IL-3的生物学效应是通过由IL3RA亚基和IL3RB信号转导亚基组成的受体复合物介导的，从而导致JAK2激酶活性的快速激活以及随后STAT5介导的转录编程。此外，在非造血系统中，IL-3 通过激活涉及 PI3K/AKT 和 ERK 的途径，有助于氧化应激期间细胞的存活。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.15 ng/mL. The specific activity of recombinant human IL-3 is approximately >1.2 x 10⁶ IU/mg

Human

E. coli

C-6*His

AAC08706.1 (A20-F152)

3562

Partial

MAPMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF

Approximately 13 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.