Animal-Free RANKL/TNFSF11 蛋白, Human (His)

RANKL/TNFSF11 protein is a cytokine that binds to TNFRSF11B/OPG and TNFRSF11A/RANK and serves as a key regulator of osteoclast differentiation and activation. In addition, it is a factor that enhances the ability of dendritic cells to stimulate naive T cell proliferation, indicating its importance in regulating the interaction between T cells and dendritic cells, as well as its potential role in T cell-dependent immune responses. Animal-Free RANKL/TNFSF11 Protein, Human (His) is the recombinant human-derived animal-FreeRANK L/TNFSF11 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

RANK L/TNFSF11 蛋白是一种细胞因子，可与 TNFRSF11B/OPG 和 TNFRSF11A/RANK 结合，作为破骨细胞分化和激活的关键调节因子。此外，它还是增强树突状细胞刺激幼稚 T 细胞增殖能力的因子，表明它在调节 T 细胞和树突状细胞相互作用中的重要性，以及在 T 细胞依赖性免疫反应中的潜在作用。此外，RANK L/TNFSF11 可能在恶性肿瘤体液高钙血症等疾病中观察到的骨吸收增强中发挥重要作用。其诱导破骨细胞生成涉及破骨细胞前体细胞中多种信号通路的激活，其关键机制是诱导细胞内钙离子浓度的长期振荡，从而导致NFATC1的激活。该转录因子易位至细胞核并启动破骨细胞特异性基因转录，促进破骨细胞分化。在此过程中，RANK L/TNFSF11 以 TMEM64 和 ATP2A2 依赖性方式诱导 CREB1 激活和线粒体 ROS 生成，这对于破骨细胞的正常形成至关重要。RANK L/TNFSF11 作为同源三聚体存在，可与 TNFRSF11B、TNFRSF11A、FBN1 和 TNFAIP6 相互作用，凸显其在不同细胞环境中的多功能相互作用。

Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED₅₀ for this effect is <10 ng/mL.

Human

E. coli

C-6*His

O14788 (E143-D317)

8600  [NCBI]

Partial

MEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID

Approximately 17 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.