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Apolipoprotein H/APOH 蛋白, Human (HEK293, His)

目录号: HY-P7533
COA 产品使用指南

Apolipoprotein H 蛋白, Human (HEK293, His)) 在 HEK293 细胞中表达,N 端带有一个 His 标签。Apolipoprotein H 是在许多系统性红斑狼疮和抗磷脂综合征 (APS) 患者中发现的抗磷脂抗体 (aPLs) 的主要靶标。

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5 μg ¥400 In-stock
10 μg ¥670 In-stock
50 μg ¥1880 In-stock
100 μg ¥3200 In-stock
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描述

Apolipoprotein H Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein H (ApoH) is the main target of anti-phospholipid antibodies (aPLs) found in many patients with systemic lupus erythematosus and antiphospholipid syndrome (APS)[1].

研究背景

载脂蛋白 H (ApoH),也称为 β2-糖蛋白 I (β2GPI),是一种 43-50 kDa 单链糖蛋白,主要在肝脏中表达,但也在其他细胞类型中表达,例如内皮细胞、淋巴细胞、星形胶质细胞和神经元。ApoH 是一种磷脂结合(例如心磷脂)血浆蛋白,还可以结合其他带负电的分子(例如 DNA 和氧化低密度脂蛋白),并与多种蛋白质相互作用[1]

生物活性

Measured in a cell proliferation assay using Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.4957 μg/mL, corresponding to a specific activity is 2.02×103 units/mg.

  • Measured in a cell proliferation assay using Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.4957 μg/mL, corresponding to a specific activity is 2.02×103 units/mg.
种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P02749 (G20-C345)

基因 ID

350  [NCBI]

蛋白结构
N-term
APOH (G20-C345)
Accession # P02749
His
C-term
同用名
rHuApolipoprotein H, His; ApoH; B2G1; B2GP1; Apolipoprotein H
氨基酸序列

GRTCPKPDDLPFSTVVPLKTFYEPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILENGAVRYTTFEYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPIICPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTKLPECREVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIECTKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSFFCKNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFWKTDASDVKPCHHHHHH

分子量

45-70 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Apolipoprotein H/APOH Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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