1. 重组蛋白
  2. Receptor Proteins Enzymes & Regulators Biotinylated Proteins
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TAM Receptor
  5. Axl Proteins Axl Proteins
  6. AXL 蛋白, Human (Biotinylated, HEK293, Fc)

AXL 蛋白, Human (Biotinylated, HEK293, Fc)

目录号: HY-P77876
产品使用指南

AXL 蛋白是一种受体酪氨酸激酶,通过与 GAS6 结合介导信号并调节细胞存活、增殖、迁移和分化。配体结合后,AXL 二聚化并自磷酸化,激活下游分子,如 PI3 激酶亚基、GRB2、PLCG1 等。AXL 蛋白, Human (Biotinylated, HEK293, Fc) 是重组的 AXL 蛋白,由 HEK293 表达,带有 C-hFc 标签。AXL 蛋白, Human (Biotinylated, HEK293, Fc) 全长 424 个氨基酸,分子量为 80-110 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

AXL protein is a receptor tyrosine kinase that mediates signals by binding to GAS6 and regulates cell survival, proliferation, migration and differentiation. After ligand binding, AXL dimerizes and autophosphorylates, activating downstream molecules, such as PI3 kinase subunits, GRB2, PLCG1, etc. AXL Protein, Human (Biotinylated, HEK293, Fc) is the recombinant human-derived AXL protein, expressed by HEK293 , with C-hFc labeled tag. The total length of AXL Protein, Human (Biotinylated, HEK293, Fc) is 424 a.a., with molecular weight of 80-110 kDa.

研究背景

AXL蛋白是一种受体酪氨酸激酶,通过结合生长因子GAS6,作为将信号从细胞外基质转导到细胞质的关键介质,从而调节细胞存活、增殖、迁移和分化等多种生理过程。细胞表面的配体结合诱导 AXL 二聚化和自磷酸化。激活后,AXL 与各种下游信号分子相互作用并诱导其酪氨酸磷酸化,包括 PI3 激酶亚基(PIK3R1、PIK3R2 和 PIK3R3)、GRB2、PLCG1、LCK、PTPN11、CBL、NCK2、SOCS1 和 TNS2。这会触发 GRB2 和磷脂酰肌醇 3 激酶调节亚基的募集,从而导致 AKT 激酶的下游激活。GAS6/AXL 信号轴在各种过程中发挥着关键作用,例如内皮细胞存活、人类自然杀伤细胞发育过程中的最佳细胞因子信号传导、肝再生、促性腺激素释放激素神经元存活和迁移、血小板激活以及血栓反应的调节。此外,AXL 还参与抑制 Toll 样受体 (TLR) 介导的先天免疫反应,并且在微生物感染的情况下,它可能通过 GAS6 与磷脂酰丝氨酸结合而充当拉沙病毒和淋巴细胞性脉络膜脑膜炎病毒的受体。病毒体包膜的表面。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P30530 (A26-P449)

基因 ID

558  [NCBI]

蛋白结构
N-term
AXL (A26-P449)
Accession # P30530
hFc
C-term
同用名
Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
分子量

80-110 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AXL Protein, Human (Biotinylated, HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AXL Protein, Human (Biotinylated, HEK293, Fc)
目录号:
HY-P77876
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