B7-H4 蛋白, Human (Biotinylated, HEK293, His-Avi)

The B7-H4 protein acts as a negative regulator, inhibiting T cell-mediated immune responses, including activation, proliferation, cytokine production, and cytotoxicity. When expressed on tumor macrophages, it cooperates with regulatory T cells to suppress tumor-associated antigen-specific T cell immunity. B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived B7-H4 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) is 230 a.a., with molecular weight of 60-70 kDa.

B7-H4 蛋白作为 T 细胞介导的免疫反应的负调节因子，对 T 细胞活化、增殖、细胞因子产生和细胞毒性的发展发挥抑制作用。当在肿瘤巨噬细胞的细胞表面表达时，B7-H4 与调节性 T 细胞 (Treg) 合作，在抑制肿瘤相关抗原特异性 T 细胞免疫方面发挥着至关重要的作用。此外，B7-H4 还参与促进上皮细胞转化，表明其多方面参与调节免疫反应和与肿瘤发展相关的细胞过程。

Measured by its binding ability in a functional ELISA. When immobilized Anti-B7-H4 Antibody at 1μg/ml (100μl/well), can bind Biotinylated Human B7-H4, His Tag and the EC₅₀ is < 65 ng/ml.

Human

HEK293

C-Avi;C-His

Q7Z7D3 (F29-A258)

79679  [NCBI]

60-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.