BRCA1 蛋白, Human

BRCA1 Protein, Human is the recombinant human-derived BRCA1, expressed by E. coli, with tag free labeled tag.

BRCA1 是一种 E3 ubiquitin-protein ligase，特异性介导 'Lys-6' 连接的 polyubiquitin 链的形成，并通过促进细胞对 DNA 损伤的反应在 DNA 修复中发挥核心作用。目前尚不清楚它是否还介导其他类型的 polyubiquitin 链的形成。BRCA1-BARD1 异二聚体协调多种细胞通路，如 DNA 损伤修复、泛素化和转录调控，以维持基因组稳定性。此外，它还调控中心体微管成核，并在细胞周期的 S 期和 G2 期电离辐射后调控适当的细胞周期停滞。BRCA1 还参与 FANCD2 向 DNA 损伤位点的靶向，通过结合非活性磷酸化的 ACACA 抑制脂质合成，并通过与 PALB2 的直接相互作用促进 homologous recombination repair (HRR)。作为 BRCA1-RBBP8 复合物的组分，它通过 BRCA1 介导的 RBBP8 泛素化调控 CHEK1 的激活，并控制 DNA 损伤后的 G2/M 检查点。BRCA1 还具有转录激活功能。

Human

E. coli

Tag Free

P38398 (V1646-P1859)

672

Partial

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.