1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens Biotinylated Proteins
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins B Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. TNF Receptor Superfamily CD40 GITR/CD357
  5. CD40 蛋白, Human (Biotinylated, HEK293, Fc-Avi)

CD40 蛋白, Human (Biotinylated, HEK293, Fc-Avi)

目录号: HY-P72909
产品使用指南

CD40 蛋白是一种 TNFSF5/CD40LG 受体,通过 TRAF6 和 MAP3K8 介导的途径转导信号,激活巨噬细胞和 B 细胞中的 ERK,并诱导免疫球蛋白分泌。CD40 以单体和同二聚体形式存在,并与 TRAF 蛋白(TRAF1、TRAF2、TRAF3、TRAF5 和 TRAF6)相互作用。CD40 蛋白, Human (Biotinylated, HEK293, Fc-Avi) 是重组的 CD40 蛋白,由 HEK293 表达,带有 C-Avi, C-hFc 标签。CD40 蛋白, Human (Biotinylated, HEK293, Fc-Avi) 全长 173 个氨基酸,分子量约为 ~47.7 kDa。

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描述

CD40 protein is a TNFSF5/CD40LG receptor that transduces signals through TRAF6 and MAP3K8-mediated pathways, activates ERK in macrophages and B cells, and induces immunoglobulin secretion. CD40 exists as monomers and homodimers and interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6). CD40 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived CD40 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of CD40 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 173 a.a., with molecular weight of ~47.7 kDa.

研究背景

CD40 蛋白作为 TNFSF5/CD40LG 的受体,有助于通过 TRAF6 和 MAP3K8 介导的途径转导信号,从而激活巨噬细胞和 B 细胞中的 ERK,并随后诱导免疫球蛋白分泌。CD40 蛋白以单体和同型二聚体形式存在,其同型二聚体结构表现出变化,如在膀胱癌细胞系 Hu549 中观察到的那样。该受体与 TRAF1、TRAF2、TRAF3、TRAF5 和 TRAF6 等关键信号分子相互作用,其中关键的相互作用发生在 CD40 蛋白、TRAF6 和 MAP3K8 之间,从而在 ERK 激活中发挥关键作用。

种属

Human

表达系统

HEK293

标签

C-Avi;C-hFc

蛋白编号

P25942 (E21-R193)

基因 ID

958  [NCBI]

蛋白结构
N-term
CD40 (E21-R193)
Accession # P25942
hFc-Avi
C-term
同用名
Tumor Necrosis Factor Receptor Superfamily member 5; Bp50; CD40L Receptor; CDw40; TNFRSF5
氨基酸序列

MVRLPLQCVLWGCLLTAVHPEPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQDRLR

分子量

Approximately 47.7 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD40 Protein, Human (Biotinylated, HEK293, Fc-Avi)
目录号:
HY-P72909
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