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  3. Cerberus 1/CER1 蛋白, Human (sf9, His)

Cerberus 1/CER1 蛋白, Human (sf9, His)

目录号: HY-P75350
产品使用指南

Cerberus 1/CER1 蛋白作为一种细胞因子出现,可能参与胚胎发生过程中的神经前诱导和体节形成,部分是通过其对 BMP 信号传导的抑制作用实现的。它的调节作用延伸到原肠胚形成,在那里它可以影响节点信号传导并有助于原条的形成和图案化。Cerberus 1/CER1 蛋白, Human (sf9, His) 是重组的 Cerberus CER1 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。Cerberus 1/CER1 蛋白, Human (sf9, His) 全长 267 个氨基酸,分子量约为 ~29.6 kDa。

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描述

The Cerberus 1/CER1 protein emerges as a cytokine that may be involved in preneural induction and somite formation during embryogenesis, in part through its inhibitory effect on BMP signaling. Its regulatory role extends into gastrulation, where it can influence nodal signaling and contribute to primitive streak formation and patterning. Cerberus 1/CER1 Protein, Human (sf9, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Cerberus 1/CER1 Protein, Human (sf9, His) is 267 a.a., with molecular weight of ~29.6 kDa.

研究背景

Cerberus 1 (CER1) 是一种细胞因子,在胚胎发育中具有潜在意义,特别是在前神经诱导和体节形成中。其参与至少部分归因于其对 BMP(骨形态发生蛋白)的抑制作用。CER1 在原肠胚形成期间的节点信号传导中表现出调节作用,并有助于原条的形成和模式化,表明其对早期发育过程的多方面影响。从结构上看,CER1 已知形成单体并主要以二聚体形式存在,表明其动态分子组织。CER1 调节关键信号通路和参与胚胎发生的能力强调了其在协调复杂发育事件中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

O95813 (M1-A267)

基因 ID
蛋白结构
N-term
CER1 (M1-A267)
Accession # O95813
His
C-term
同用名
Cerberus; DAN domain family member 4; CER1; DAND4
分子量

Approximately 29.6 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Cerberus 1/CER1 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Cerberus 1/CER1 Protein, Human (sf9, His)
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HY-P75350
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