CISD1 蛋白, Human (His)

The CISD1 protein acts as an L-cysteine aminotransferase, mediating the reversible transfer between L-cysteine and 2-oxoglutarate. Facilitated by the pyridoxal 5'-phosphate (PLP) cofactor, this process produces 2-oxo-3-sulfanylpropionate and L-glutamic acid. CISD1 Protein, Human (His) is the recombinant human-derived CISD1 protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of CISD1 Protein, Human (His) is 77 a.a., with molecular weight of ~14 KDa.

CISD1 蛋白充当 L-半胱氨酸转氨酶，促进氨基从 L-半胱氨酸可逆转移至 α-酮酸 2-酮戊二酸。该酶促过程导致 2-氧代-3-硫烷基丙酸酯和 L-谷氨酸的形成。催化循环是在吡哆醛 5'-磷酸 (PLP) 辅因子存在下精心策划的，该辅因子通过与酶上活性位点 Lys-55 残基的ε-氨基形成内部醛亚胺来启动转氨作用（PLP-酶醛亚胺） ）。该内部醛亚胺随后被具有底物氨基的外部醛亚胺（PLP-L-半胱氨酸醛亚胺）的形成所取代。外部醛亚胺发生去质子化，导致碳负离子中间体的形成。在 2-氧代戊二酸存在下，该中间体会再生 PLP，最终产生最终产品 2-氧代-3-硫烷基丙酸和 L-谷氨酸。活性位点赖氨酸残基参与控制碳负离子中间体中的质子转移，而PLP通过电子离域稳定碳负离子结构，这种现象称为电子汇效应。此外，CISD1 在调节电子传输和氧化磷酸化的最大容量方面发挥着至关重要的作用，并且可能参与铁硫簇穿梭和/或氧化还原反应。值得注意的是，它可以将 [2Fe-2S] 簇转移到脱辅基受体蛋白上，特别是在氧化应激条件下，这表明它可以作为氧化还原传感器调节线粒体铁硫簇组装和铁运输。

Human

E. coli

N-His;N-6*His

Q9NZ45/NP_060934.1 (K32-T108)

55847  [NCBI]

KRFYVKDHRNKAMINLHIQKDNPKIVHAFDMEDLGDKAVYCRCWRSKKFPFCDGAHTKHNEETGDNVGPLIIKKKET

Approximately 14 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.