COMP 蛋白, Human (HEK293, His)

The COMP protein is critical for cartilage integrity and interacts with extracellular matrix proteins to promote the connection of chondrocytes to the cartilage matrix through integrin receptors. Its role in the pathogenesis of osteoarthritis emphasizes the significance of joint health. COMP Protein, Human (HEK293, His) is the recombinant human-derived COMP protein, expressed by HEK293 , with C-His labeled tag. The total length of COMP Protein, Human (HEK293, His) is 737 a.a., with molecular weight of 120-130 kDa.

COMP 蛋白通过与其他细胞外基质蛋白（包括胶原蛋白和纤连蛋白）相互作用，在保持软骨结构完整性方面发挥着至关重要的作用。它通过与细胞表面整合素受体结合来促进软骨细胞和软骨细胞外基质之间的连接。此外，COMP 参与骨关节炎的发病机制强调了其对关节健康的潜在重要性。除了其结构作用之外，COMP 还作为原代软骨细胞和转化细胞中细胞凋亡的有效抑制剂。其抗凋亡作用是通过抑制 caspase-3 激活和诱导 IAP 存活蛋白家族（BIRC3、BIRC2、BIRC5 和 XIAP）来介导的。此外，COMP通过与ITGA7的相互作用，在生理和病理刺激下维持血管平滑肌细胞的收缩和分化表型方面发挥着重要作用。这种多方面的功能使 COMP 成为维持软骨结构、关节健康和细胞生存过程的关键角色。

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to COMP-coated plates (0.625 μg/mL and 100 μL/well), approximately ≥30% cells will adhere specifically after 60 minutes at 37°C.

Human

HEK293

C-His

P49747 (Q21-A757)

1311  [NCBI]

MVPDTACVLLLTLAALGASGQGQSPLGSDLGPQMLRELQETNAALQDVRELLRQQVREITFLKNTVMECDACGMQQSVRTGLPSVRPLLHCAPGFCFPGVACIQTESGARCGPCPAGFTGNGSHCTDVNECNAHPCFPRVRCINTSPGFRCEACPPGYSGPTHQGVGLAFAKANKQVCTDINECETGQHNCVPNSVCINTRGSFQCGPCQPGFVGDQASGCQRRAQRFCPDGSPSECHEHADCVLERDGSRSCVCAVGWAGNGILCGRDTDLDGFPDEKLRCPERQCRKDNCVTVPNSGQEDVDRDGIGDACDPDADGDGVPNEKDNCPLVRNPDQRNTDEDKWGDACDNCRSQKNDDQKDTDQDGRGDACDDDIDGDRIRNQADNCPRVPNSDQKDSDGDGIGDACDNCPQKSNPDQADVDHDFVGDACDSDQDQDGDGHQDSRDNCPTVPNSAQEDSDHDGQGDACDDDDDNDGVPDSRDNCRLVPNPGQEDADRDGVGDVCQDDFDADKVVDKIDVCPENAEVTLTDFRAFQTVVLDPEGDAQIDPNWVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTESQVRLLWKDPRNVGWKDKKSYRWFLQHRPQVGYIRVRFYEGPELVADSNVVLDTTMRGGRLGVFCFSQENIIWANLRYRCNDTIPEDYETHQLRQA

120-130 kDa

Greater than 80% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.