1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. CPS1/CPSase I 蛋白, Human (His)

CPS1/CPSase I 蛋白, Human (His)

目录号: HY-P71445
产品使用指南

CPS1/CPSase I 蛋白对于参与尿素循环至关重要,对于消除携带尿素的动物中过量的氨至关重要。这种必需的酶将有毒的氨(氨基酸分解代谢的副产品)转化为尿素,促进安全排泄。CPS1/CPSase I 蛋白, Human (His) 是重组的 CPS1/CPSase I 蛋白,由 E. coli 表达,带有 N-His 标签。CPS1/CPSase I 蛋白, Human (His) 全长 147 个氨基酸,分子量约为 ~20.5 kDa。

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描述

The CPS1/CPSase I protein is critical in participating in the urea cycle and is essential for the elimination of excess ammonia in urea-bearing animals. This essential enzyme converts toxic ammonia (a byproduct of amino acid catabolism) into urea, promoting safe excretion. CPS1/CPSase I Protein, Human (His) is the recombinant human-derived CPS1/CPSase I protein, expressed by E. coli , with N-His labeled tag. The total length of CPS1/CPSase I Protein, Human (His) is 147 a.a., with molecular weight of ~20.5 kDa.

研究背景

CPS1/CPSase I 蛋白是尿素循环的关键参与者,特别是在尿素动物中,它在消除细胞中多余的氨方面发挥着至关重要的作用。作为这一代谢途径中的必需酶,CPS1 有助于将氨(氨基酸分解代谢的有毒副产品)转化为尿素,从而可以安全地从体内排出。这种酶活性对于维持氮平衡和防止有害水平的氨积累至关重要,突显了 CPS1 在尿素循环和尿素生物整体氮代谢中不可或缺的作用。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P31327 (1354G-1500A)

基因 ID
蛋白结构
N-term
His
CPS1 (1354G-1500A)
Accession # P31327
C-term
同用名
Carbamoyl phosphate synthase; Carbamoyl phosphate synthase mitochondrial; Carbamoyl phosphate synthase; Carbamoyl phosphate synthetase 1; Carbamoyl phosphate synthetase 1 mitochondrial; Carbamoyl phosphate synthetase I; Carbamoyl-phosphate synthase [ammonia]; Carbamoyl-phosphate synthetase I; Carbamoylphosphate synthase; Carbamoylphosphate synthetase 1; Carbamoylphosphate synthetase I; CPS 1; Cps1; CPSase 1; CPSase I; CPSASE1; mitochondrial; MS738
氨基酸序列

GFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQYSAGKAA

分子量

Approximately 20.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CPS1/CPSase I Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CPS1/CPSase I Protein, Human (His)
目录号:
HY-P71445
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