1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors Cystatin Family
  4. Cystatin C
  5. Cystatin C/CST3 蛋白, Mouse (HEK293, His)

Cystatin C/CST3 蛋白, Mouse (HEK293, His)

目录号: HY-P72966
COA 产品使用指南

半胱氨酸蛋白酶抑制剂 C (CST3) 是一种半胱氨酸蛋白酶抑制剂,可作为局部调节剂,通过抑制特定细胞环境中的半胱氨酸蛋白酶来调节蛋白水解过程,从而发挥至关重要的作用。Cystatin C/CST3 蛋白, Mouse (HEK293, His) 是重组的 胱抑素 C/CST3 蛋白,由 HEK293 表达,带有 C-His 标签。Cystatin C/CST3 蛋白, Mouse (HEK293, His) 全长 140 个氨基酸,分子量分别约为 ~15 & 18 & 21-24 kDa。

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规格 价格 是否有货 数量
20 μg ¥2450 In-stock
100 μg ¥6800 询价
> 100 μg   询价  

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描述

Cystatin C (CST3), an inhibitor of cysteine proteinases, acts as a local regulator, playing a vital role in modulating proteolytic processes by inhibiting cysteine proteinases within specific cellular environments. Cystatin C/CST3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Cystatin C/CST3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Cystatin C/CST3 Protein, Mouse (HEK293, His) is 140 a.a., with molecular weight of ~15 & 18 & 21-24 kDa, respectively.

研究背景

半胱氨酸蛋白酶抑制剂 C (CST3) 作为半胱氨酸蛋白酶的抑制剂,被认为作为该酶活性的局部调节剂发挥着至关重要的生理作用。其抑制功能有助于半胱氨酸蛋白酶的调节,表明其在调节特定细胞环境中的蛋白水解过程中发挥作用。

生物活性

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC and the IC50 value is < 25 nM.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P21460 (M1-A140)

基因 ID

13010  [NCBI]

蛋白结构
N-term
CST3 (M1-A140)
Accession # P21460
His
C-term
同用名
Cystatin-C; Cystatin-3; Cst3
氨基酸序列

MASPLRSLLFLLAVLAVAWAATPKQGPRMLGAPEEADANEEGVRRALDFAVSEYNKGSNDAYHSRAIQVVRARKQLVAGVNYFLDVEMGRTTCTKSQTNLTDCPFHDQPHLMRKALCSFQIYSVPWKGTHSLTKFSCKNA

分子量

Approximately 15&18&21-24 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cystatin C/CST3 Protein, Mouse (HEK293, His)
目录号:
HY-P72966
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