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  3. EEF1B2 蛋白, Human (His)

EEF1B2 蛋白, Human (His)

目录号: HY-P76898
COA 产品使用指南

EEF1B2 蛋白促进 EEF1A 蛋白上的 GDP 到 GTP 的交换,从而推动转化。EEF1 包含四个亚基:α、β、δ 和γ。EEF1B2 蛋白, Human (His) 是重组的 EEF1B2 蛋白,由 E. coli 表达,带有 N-6*His 标签。EEF1B2 蛋白, Human (His) 全长 224 个氨基酸,分子量约为 ~29 kDa。

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5 μg ¥650 In-stock
10 μg ¥1050 In-stock
50 μg ¥3000 In-stock
100 μg ¥5000 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

EEF1B2 Protein facilitates GDP-to-GTP exchange on EEF1A Protein, driving the conversion. EEF1 comprises four subunits: alpha, beta, delta, and gamma. EEF1B2 Protein, Human (His) is the recombinant human-derived EEF1B2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of EEF1B2 Protein, Human (His) is 224 a.a., with molecular weight of ~29 kDa.

研究背景

EEF1B2 蛋白催化与 EEF1A 蛋白结合的 GDP 的交换,促进向 GTP 的转化。EEF1 由四个亚基组成:α、β、δ 和γ。

生物活性

Data is not available.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P24534 (G2-I225)

基因 ID
蛋白结构
N-term
6*His
EEF1B2 (G2-I225)
Accession # P24534
C-term
同用名
Elongation factor 1-beta; EF-1-beta; EEF1B; EF1B
氨基酸序列

GFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI

分子量

Approximately 29 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

EEF1B2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EEF1B2 Protein, Human (His)
目录号:
HY-P76898
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