Ephrin-A1/EFNA1 蛋白, Rat (HEK293, His)

The Ephrin-A1/EFNA1 protein is a cell surface GPI-binding ligand of the Eph receptor and is involved in migration, repulsion, and adhesion during development. Ephrin-A1/EFNA1 Protein, Rat (HEK293, His) is the recombinant rat-derived Ephrin-A1/EFNA1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Ephrin-A1/EFNA1 Protein, Rat (HEK293, His) is 164 a.a., with molecular weight of ~23 kDa.

Ephrin-A1/EFNA1 蛋白是 Eph 受体的细胞表面 GPI 结合配体，在神经元、血管和上皮发育过程中协调迁移、排斥和粘附中发挥着至关重要的作用。作为混杂结合剂，Ephrin-A1 与邻近细胞上的 Eph 受体结合，促进接触依赖性双向信号传导。其重要性延伸至血管生成和肿瘤新生血管形成，其中磷酸化 EPHA2 募集 VAV2、VAV3 和 PI3 激酶 p85 亚基对于 EFNA1 诱导的 RAC1 GTPase 激活、血管内皮细胞迁移和组装至关重要。此外，Ephrin-A1 通过激活和下调 EPHA2，诱导其内化和降解来发挥抗癌作用。在神经胶质瘤中，Ephrin-A1 充当负调节因子，下调 EPHA2 和 FAK，从而减轻肿瘤发生过程。除了在血管生成和肿瘤发生中的作用外，Ephrin-A1 还可以引起胚胎神经元生长锥的塌陷并调节树突棘形态发生。Ephrin-A1 以单体或同二聚体形式存在，与 EPHA2 形成异二聚体，并与一系列受体酪氨酸激酶（包括 EPHA1）结合，强调了其多样化的分子相互作用。

Measured by its binding ability in a functional ELISA. Immobilized Ephrin-A1 at 10μg/mL (100 μL/well) can bind biotinylated EPHA2 Protein. The ED₅₀ for this effect is 4.986 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Ephrin-A1 at 10 μg/mL (100 μL/well) can bind biotinylated EPHA2 Protein. The ED₅₀ for this effect is 4.986 ng/mL.

Rat

HEK293

C-His

P97553 (A18-H181)

94268  [NCBI]

ADRHIVFWNSSNPKFREEDYTVHVQLNDYLDIICPHYEDDSVADAAMERYTLYMVEHQEYVTCEPQSKDQVRWKCNQPSAKHGPEKLSEKFQRFTPFTLGKEFKEGHSYYYISKPIYHQETQCLKLKVTVNGKITHSPHAHANPQEKRLQADDPEVQVLHSIGH

Approximately 23 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.