FGF-21 蛋白, Human (HEK293, mFc-Avi)

FGF-21 Proteinas, pivotal in promoting glucose uptake in adipocytes, induces SLC2A1/GLUT1 expression, not affecting SLC2A4/GLUT4, contingent on KLB. It significantly regulates systemic glucose homeostasis and insulin sensitivity, with direct KLB interaction via its C-terminus. Engagement with FGFR4 adds complexity to its molecular mechanisms, highlighting its role in orchestrating cellular responses beyond localized effects. FGF-21 Protein, Human (HEK293, mFc-Avi) is the recombinant human-derived FGF-21 protein, expressed by HEK293 , with N-mFc, N-Avi labeled tag. The total length of FGF-21 Protein, Human (HEK293, mFc-Avi) is 181 a.a., with molecular weight of 52-60 kDa.

FGF-21 蛋白通过特异性诱导葡萄糖转运蛋白 SLC2A1/GLUT1 的表达，同时不影响 SLC2A4/GLUT4 的表达，在促进分化脂肪细胞内的葡萄糖摄取方面发挥关键作用。其活动取决于 KLB 的存在。除了其局部作用外，这种蛋白质还对全身葡萄糖稳态和胰岛素敏感性的调节做出了重大贡献。通过其 C 末端促进与 KLB 的直接相互作用，强调了其功能的分子基础。此外，该蛋白质与 FGFR4 结合，进一步凸显了其在协调细胞反应中相互作用的复杂性。

Immobilized Human FGF21 at 5 μg/mL (100 μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC₅₀ of 2.29 μg/mL determined by ELISA.

Human

HEK293

N-mFc;N-Avi

Q9NSA1 (H29-S209)

26291  [NCBI]

52-60 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.