FGF-21 蛋白, Human (His)

FGF-21 Proteinas influences glucose uptake in adipocytes by inducing SLC2A1/GLUT1 expression, particularly with KLB's presence. It plays a crucial role in systemic glucose homeostasis and insulin sensitivity, interacting directly with KLB through its C-terminus and engaging with FGFR4. The protein's molecular mechanisms involve a complex interplay, emphasizing its broad impact beyond localized effects. FGF-21 Protein, Human (His) is the recombinant human-derived FGF-21 protein, expressed by E. coli , with N-6*His labeled tag. The total length of FGF-21 Protein, Human (His) is 181 a.a., with molecular weight of ~23.0 kDa.

FGF-21 蛋白通过促进分化脂肪细胞中的葡萄糖摄取来发挥其生物学影响，主要是通过诱导葡萄糖转运蛋白 SLC2A1/GLUT1，而不是 SLC2A4/GLUT4 的表达。这项活动可能取决于 KLB 的存在。除了其局部作用之外，FGF-21 在调节全身葡萄糖稳态和胰岛素敏感性方面也发挥着至关重要的作用。该蛋白通过其 C 末端直接与 KLB 相互作用，并且还与 FGFR4 结合，表明其分子机制中存在复杂的相互作用。

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ this effect is 1.756 μg/mL in the presence of 1.25 μg/mL, recombinant human Klotho beta. Corresponding to a specific activity is 569.4761 units/mg.

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  Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ this effect is 1.756 μg/mlin the presence of 1.25 μg/ml, recombinant human Klotho beta. Corresponding to a specific activity is 569.4761 units/mg.

Human

E. coli

N-6*His

AAH18404.1 (H29-S209)

26291  [NCBI]

HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS

Approximately 23.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 100 mM NaCl, 2 mM EDTA, pH 9.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.