Glucokinase/GCK 蛋白, Human

Glucokinase/GCK proteins catalyze hexose phosphorylation, converting D-glucose, D-fructose, and D-mannose into their respective hexose 6-phosphates. Glucokinase/GCK Protein, Human is the recombinant human-derived Glucokinase/GCK protein, expressed by E. coli , with tag free. The total length of Glucokinase/GCK Protein, Human is 464 a.a., with molecular weight of ~55 kDa.

葡萄糖激酶 (GCK) 蛋白是葡萄糖代谢的关键参与者，催化 D-葡萄糖、D-果糖和 D-甘露糖等己糖磷酸化，形成 6-磷酸己糖，特别是 D-葡萄糖 6-磷酸，D-果糖6-磷酸和D-甘露糖6-磷酸。值得注意的是，与其他己糖激酶相比，GCK 对 D-葡萄糖的亲和力较弱，并且在葡萄糖丰富时最有效。它在胰腺β细胞和肝脏中的主要表达使GCK成为这些组织内葡萄糖代谢的限速步骤。GCK 作为胰腺 β 细胞中的葡萄糖传感器，其低葡萄糖亲和力使其能够与葡萄糖浓度协调调节胰岛素分泌。在肝脏中，GCK 在促进葡萄糖摄取和转化方面发挥着至关重要的作用，是肝葡萄糖使用的胰岛素敏感决定因素。此外，GCK 对于在糖原合成中提供 D-葡萄糖 6-磷酸至关重要，并通过催化 D-葡萄糖磷酸化为 D-葡萄糖 6-磷酸来介导糖酵解的初始步骤。

Specific activity is 2022.238 pmol/min/µg. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH 8.0 at 37°C.

Human

E. coli

Tag Free

P35557-1 (L2-Q465)

2645  [NCBI]

LDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Approximately 55 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.