1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-gamma Receptor
  5. IFN-gamma R2
  6. IFN-gamma R2 蛋白, Human (CHO)

IFN-gamma R2 蛋白, Human (CHO)

目录号: HY-P7367
COA 产品使用指南

IFN-gamma R2,IFN-gamma 受体的亚基之一,是 IFN-γ 的结合受体。IFN-gamma R2 是 IFN-gamma 受体的信号转导链,可以与 IFN-gamma R1 结合形成功能性受体。与配体 IFN-γ 结合后,IFN-gamma R2 和 IFN-gamma R1 寡聚化并发生磷酸化。然后,下游的信号分子 JAK1 和 JAK2 发生磷酸化并激活,STAT1 会发生磷酸化。磷酸化的 STAT1 易位至细胞核,从而调节 IFN-反应性的基因 (例如 CD54) 的表达。IFN-gamma R2 蛋白, Human (CHO) 是一种由 CHO 细胞产生的重组人 IFN-gamma R2 (S28-Q247),不带标签。

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规格 价格 是否有货 数量
10 μg ¥800 In-stock
50 μg ¥2500 In-stock
100 μg   询价  
250 μg   询价  

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描述

IFN-gamma R2, one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R2 is the signal-transducing chain of the IFN-gamma receptor. IFN-gamma R2 forms the functional receptor with IFN-gamma R1. Upon binding with IFN-gamma, IFN-gamma R2 and IFN-gamma R1 oligomerize and transphosphorylate[1]. Then, the downstream signaling components JAK1 and JAK2 are phosphorylated and activated, and STAT1 is phosphorylated. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)[2]. IFN-gamma R2 Protein, Human (CHO) is a recombinant human IFN-gamma R2 (S28-Q247) without any tag, which is produced in CHO cells.

研究背景

IFN-gamma R2, one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R2. IFN-gamma R2 is highly expressed on myeloid cells, moderately on B cells and low on T cells, and the expression is regulated by the state of cellular differentiation or activation[2].
IFN-gamma R2 can associate with IFN-gamma R1 to form a functional receptor. Upon binding with IFN-gamma, IFN-gamma R2 and IFN-gamma R1 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, creating a binding site for STAT1. STAT1 is recruited to the receptor complex and leads to the phosphorylation. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54). IFN-gamma R2 is important for innating immune defense against mycobacterial infections. Defects in IFN-gamma R2 causes mendelian susceptibility to mycobacterial disease (MSMD)[2].
Human IFN-gamma R2 consists of extracellular domain (S28-Q247), helical domain (V248-F268), and cytoplasmic domain (L269-L337).
IFN-gamma R2 plays a critical role in antimicrobial, and immune responses[2].

生物活性

The ED50 is <0.1 µg/mL as measured by HT-29 (HTB-38) cells.

种属

Human

表达系统

CHO

标签

Tag Free

蛋白编号

P38484 (S28-Q247)

基因 ID

3460  [NCBI]

中文名
重组人 IFN-gamma R2 蛋白 (CHO)
同用名
rHuIFN-γ Receptor II; IFNGR2; IFNGT1
氨基酸序列

SQLPAPQHPKIRLYNAEQVLSWEPVALSNSTRPVVYQVQFKYTDSKWFTADIMSIGVNCTQITATECDFTAASPSAGFPMDFNVTLRLRAELGALHSAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTAFFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWNKSNIFRVGHLSNISCYETMADASTELQQ

分子量

38-40 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-gamma R2 Protein, Human (CHO)
目录号:
HY-P7367
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