IgG4 Fc 蛋白, Human (HEK293)

The IgG4 Fc is the constant region of the immunoglobulin heavy chain, which forms the backbone of antibodies crucial in humoral immunity. B lymphocytes produce glycoprotein antibodies that initiate clonal expansion upon antigen binding. IgG4 Fc Protein, Human (HEK293) is the recombinant human-derived IgG4 Fc protein, expressed by HEK293 , with tag free.

IgG4 Fc 蛋白代表免疫球蛋白重链的恒定区，是体液免疫系统的组成部分。免疫球蛋白或抗体是 B 淋巴细胞产生的糖蛋白。在体液免疫中，膜结合免疫球蛋白充当受体，在抗原结合后启动 B 淋巴细胞的克隆扩增和分化为浆细胞。然后分泌的免疫球蛋白执行效应阶段，导致结合抗原的消除。每个免疫球蛋白具有两个由一条重链及其相关轻链的可变结构域形成的抗原结合位点。这些可变结构域经历 V-(D)-J 重排和体细胞超突变，从而实现特定抗原的亲和力成熟。在结构上，免疫球蛋白由通过二硫键连接的两条相同的重链和两条相同的轻链组成。这种复杂的分子结构强调了它们在免疫识别和反应中的关键作用。

sIgG4 Fc immobilized on CM5 Chip can bind FCRN-B2M Heterodimer with an affinity constant of 944.2 nM as determined in SPR assay.

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  sIgG4 Fc immobilized on CM5 Chip can bind FCRN-B2M Heterodimer with an affinity constant of 944.2 nM as determined in SPR assay.

Human

HEK293

Tag Free

P01861-1 (E99-G326)

3503  [NCBI]

Partial

ESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLG

Approximately 30-35 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.