1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-17
  5. IL-17F
  6. IL-17F 蛋白, Human (HEK293)

IL-17F 蛋白, Human (HEK293)

目录号: HY-P72586
COA 产品使用指南

IL-17F 是一种炎性同型二聚体细胞因子,可诱导许多促炎细胞因子和趋化因子。IL-17F 也能诱导抗菌肽。IL-17F 在结肠癌发展中起保护作用,可用于自身免疫性疾病、感染和癌症的研究。IL-17F 蛋白, Human (HEK293) 是一种重组人 IL-17F 蛋白,在 HEK293 中表达,由 133 个氨基酸组成(R31-Q163)。

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50 μg ¥5050 In-stock
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描述

IL-17F is an inflammatory homodimeric cytokine that induces many proinflammatory cytokines and chemokines. IL-17F also induces antimicrobial peptides. IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer[1][2]. IL-17F Protein, Human (HEK293) is a recombinant human IL-17F protein and is expressed in HEK293 cells. It consists of 133 amino acids (R31-Q163).

研究背景

白细胞介素-17F (IL-17F) 属于 IL-17 细胞因子家族。IL-17F 在活化的 CD4 T 细胞、活化的单核细胞、嗜碱性粒细胞和肥大细胞中表达。IL-17F 可由分化的 TH17 细胞、固有层 T 细胞、记忆 CD4+ T 细胞、γδ T 细胞和 NKT 细胞[1]产生。
人IL-17F与小鼠的氨基酸序列同一性为55.90%,与大鼠的氨基酸序列同一性为57.14%。
IL-17F是一种炎症细胞因子,可诱导多种促炎细胞因子和趋化因子,包括TGF-β、IL-2、ICAM1、GM-CSF、CCL2、CCL7、TSLP、MMP13、IL-6和CXCL1。IL-17F还与IL-22一起诱导抗菌肽,包括hBD-2、S100A7、S100A8和S100A9,并且可以与人嗜酸性粒细胞中的IL-23协同作用,促进IL-1β和IL-6的产生。IL-17F 是同型二聚体细胞因子。IL-17FIL-17A 最相似(50% 同源性),并且可以作为 IL-17AF 异二聚体产生。IL-17A、IL-17F和IL-17A/F使用相同的受体复合物:IL-17RA和IL-17RC异二聚体。它们触发性质相似的信号通路,并且 IL-17F 表现出最低的生物活性。IL-17FIL-17RA 亚基的亲和力比 IL-17A 低约 100–1000 倍,并且不与 IL-17A 竞争与 IL-17RA 的结合[1][2]
IL-17F 在结肠癌发展中发挥保护作用,可用于自身免疫性疾病、感染和癌症的研究[1][3][4]

体内研究
(In Vivo)

IL-17F (50 ng/mL; 16-24 h) induces GRO-α, IL-6, and IL-8 secretion in human primary foreskin fibroblast (BJ) cells[5].
IL-17F binds weakly to IL-17RA.Fc with an EC50 > 2000 ng/mL, whereas IL-17A binds IL-17RA.Fc with a value of 19 ng/mL, the IL-17F/IL-17A heterodimer has an EC50 of 329 ng/mL[5].
IL-17F (0-150 ng/mL; 0-72 h) increases the cell viability of PC-3 and DU145[6].
IL-17F (100 ng/mL; 24 h) stimulates the malignant phenotypes of PCa cells and activates PI3K/AKT signalling pathway in PCa cells[6].

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

AAH70124.1 (R31-Q163)

基因 ID
蛋白结构
N-term
IL-17F (R31-Q163)
Accession # AAH70124.1
C-term
中文名
重组人白细胞介素 17F 蛋白 (HEK293)
同用名
Interleukin-17F; IL-17F; Cytokine ML-1; Interleukin-24; IL-24; IL17F; IL24
氨基酸序列

RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ

分子量

Homodimer. Approximately 18 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-17F Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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