1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules NK Cell CD Proteins Macrophage CD Proteins
  4. LAG-3 LAG-3/CD223
  5. LAG-3 蛋白, Rat (HEK293, His)

LAG-3 蛋白, Rat (HEK293, His)

目录号: HY-P74788
产品使用指南

LAG-3(淋巴细胞激活基因3)蛋白是抗原激活T细胞上的抑制性受体,通过与FGL1等配体结合来传递抑制信号。LAG-3 与免疫突触处的 CD3-TCR 相关,阻碍 T 细胞激活,并可能与 PDCD1/PD-1 配合,充当 PD-1 的共受体。LAG-3 蛋白, Rat (HEK293, His) 是重组的 LAG-3 蛋白,由 HEK293 表达,带有 C-His 标签。LAG-3 蛋白, Rat (HEK293, His) 全长 419 个氨基酸,分子量约为 ~55 kDa。

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描述

LAG-3 (lymphocyte activation gene 3) protein is an inhibitory receptor on antigen-activated T cells, which transmits inhibitory signals by binding to ligands such as FGL1. LAG-3 is associated with CD3-TCR at the immune synapse, hinders T cell activation, and may cooperate with PDCD1/PD-1 to act as a co-receptor for PD-1. LAG-3 Protein, Rat (HEK293, His) is the recombinant rat-derived LAG-3 protein, expressed by HEK293 , with C-His labeled tag. The total length of LAG-3 Protein, Rat (HEK293, His) is 419 a.a., with molecular weight of ~55 kDa.

研究背景

LAG-3(淋巴细胞激活基因 3)蛋白作为抗原激活 T 细胞的抑制性受体,在与 FGL1 等配体结合后传递抑制信号,FGL1 是 LAG3 T 细胞抑制功能的主要贡献者。T 细胞受体 (TCR) 接合后,LAG3 与免疫突触中的 CD3-TCR 结合,直接阻碍 T 细胞激活。LAG3 可能与 PDCD1/PD-1 合作,潜在地充当 PD-1 的辅助受体,抑制抗原特异性 T 细胞激活。该蛋白负向调节 CD8(+) 和 CD4(+) T 细胞的增殖、激活、效应功能和稳态。此外,LAG-3 在免疫耐受中发挥着关键作用,在调节性 T 细胞 (Treg) 子集上组成型表达,有助于其抑制功能。此外,LAG-3 充当浆细胞样树突状细胞 (pDC) 激活的负调节因子,并表现出与 MHC II 类 (MHC-II) 的相互作用,尽管 MHC-II 结合的确切作用仍不清楚。LAG-3 可能作为抗原呈递细胞 (APC) 上 MHC II 类的配体发挥作用,可能促进 APC 激活/成熟并驱动 Th1 免疫反应。

种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

Q5BK54 (G24-L442)

基因 ID

297596  [NCBI]

蛋白结构
N-term
LAG-3 (G24-L442)
Accession # Q5BK54
His
C-term
同用名
Lymphocyte activation gene 3 protein; Protein FDC; CD223
分子量

Approximately 55 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LAG-3 Protein, Rat (HEK293, His)
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