MDK 蛋白, Human (sf9)

Midkine (MDK) protein is a multifunctional cytokine and growth factor that signals through cell surface proteoglycan and non-proteoglycan receptors. MDK regulates inflammatory responses, cell proliferation, adhesion, growth, survival, tissue regeneration, differentiation, and migration and plays a crucial role in inflammation by recruiting neutrophils and macrophages. MDK Protein, Human (sf9) is the recombinant human-derived MDK protein, expressed by Sf9 insect cells , with tag free. The total length of MDK Protein, Human (sf9) is 143 a.a., with molecular weight of ~18 kDa.

中期因子 (MDK) 是一种分泌蛋白，充当多功能细胞因子和生长因子，通过细胞表面蛋白聚糖和非蛋白聚糖受体传递信号。MDK 参与各种生理过程，调节炎症反应、细胞增殖、粘附、生长、存活、组织再生、分化和迁移。它通过介导中性粒细胞和巨噬细胞向炎症部位的募集而在炎症过程中发挥关键作用，表现出双重活性，包括促进上皮细胞存活和促进肾脏和血管损伤后平滑肌细胞迁移。此外，MDK 还可抑制耐受性树突状细胞的发育，抑制调节性 T 细胞分化，并通过 NFAT 信号传导和 Th1 细胞分化促进 T 细胞扩增。MDK 的参与范围扩展到组织再生，通过负调节炎症细胞募集和通过 MAPK 和 AKT 途径介导细胞存活，促进心脏损伤恢复，同时促进肝脏再生、骨修复和大脑发育。与各种受体（例如 PTPRZ1、ITGA4:ITGB1 复合物、LRP1 和 GPC2）的相互作用强调了 MDK 在多种生理过程中复杂的调节作用。

Human

Sf9 insect cells

Tag Free

P21741 (M1-D143)

4192  [NCBI]

Approximately 18 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM PBS, 1 M NaCl, pH 6.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.