OGG1 蛋白, Human (N-His)

The OGG1 protein is a DNA repair enzyme specifically designed to cleave DNA at the 8-oxoG residue and plays a key role in maintaining genome integrity. It demonstrates the ability to excise damaged bases such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine from the DNA structure (FAPY). OGG1 Protein, Human (N-His) is the recombinant human-derived OGG1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of OGG1 Protein, Human (N-His) is 344 a.a., with molecular weight of ~38.7 kDa.

OGG1（8-氧鸟嘌呤 DNA 糖基化酶 1）是一种 DNA 修复酶，对于维持基因组完整性至关重要。OGG1 专门修复 DNA 氧化损伤，在 8-oxoG（8-oxoguanine）残基处特异性切割 DNA。此外，它还能切除其他氧化损伤的碱基，例如 7,8-二氢-8-氧代鸟嘌呤和 2,6-二氨基-4-羟基-5-N-甲基甲酰胺嘧啶 (FAPY)，有助于去除 DNA 中的诱变损伤。值得注意的是，OGG1 具有 β-裂合酶活性，使其能够在病变部位的 DNA 链 3' 处产生切口，启动修复过程。这种酶的多功能性凸显了 OGG1 通过抵消氧化应激对 DNA 完整性的有害影响来保护基因组的关键作用（参考文献不可用）。

Data is not available.

Human

E. coli

N-6*His

O15527-1 (P2-G345)

4968

PARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG

Approximately 38.7 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4, 40% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.