PCSK9 蛋白, Macaca nemestrina (HEK293, His)

The PCSK9 protein controls plasma cholesterol levels by interacting with LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2 to promote their degradation. It prevents LDLR recycling and directs it to lysosomes for degradation. PCSK9 induces LDLR ubiquitination, inhibits APOB degradation, disposes of BACE1 intermediates, reduces ENaC surface expression, and regulates neuronal apoptosis through LRP8/APOER2. PCSK9 Protein, Macaca nemestrina (HEK293, His) is the recombinant cynomolgus-derived PCSK9 protein, expressed by HEK293, with C-6*His labeled tag.

PCSK9蛋白在维持血浆胆固醇水平的平衡中起着至关重要的作用。它与低密度脂蛋白受体家族成员（例如 LDLR、VLDLR、LRP1/APOER 和 LRP8/APOER2）相互作用，促进它们在细胞酸性室内的降解。它通过非蛋白水解机制，通过网格蛋白 LDLRAP1/ARH 介导的途径增强肝脏 LDLR 的降解，阻止其从内体循环至细胞表面，并将其引导至溶酶体进行降解。此外，PCSK9 可以诱导 LDLR 泛素化，导致其随后降解。它还通过影响自噬体/溶酶体途径，抑制 APOB 的细胞内降解，与 LDLR 无关。此外，PCSK9 通过处理 BACE1 的非乙酰化中间体参与早期分泌途径。它通过减少 ENaC 表面表达（主要是通过增加蛋白酶体降解）来进一步调节上皮 Na(+) 通道 (ENaC) 介导的 Na(+) 吸收。最后，PCSK9通过调节LRP8/APOER2的水平和相关的抗凋亡信号通路来调节神经元凋亡。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Others

HEK293

C-6*His

A8T662 (Q31-Q152&S153-Q692)

105495119  [NCBI]

Full Length

QEDEDGDYEELVLALRSEEDGLADAPEHGATATFHRCAKDPWRLPGTYVVVLKEETHRSQSERTARRLQAQAARRGYLTKILHVFHHLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQ&SIPWNLERITPARYRADEYQPPKGGSLVEVYLLDTSIQSDHREIEGRVMVTDFESVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGAGLRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVFNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSRSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHRAGWQLFCRTVWSAHSGPTRMATAVARCAQDEELLSCSSFSRSGKRRGERIEAQGGKRVCRAHNAFGGEGVYAIARCCLLPQVNCSVHTAPPAGASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVIVACEDGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEEAVAAVAICCRSRHLVQASQELQ

(16-21)&(55-77) kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 0.1 M Arg, 0.1 M Glu, 20% glycerol, 0.01% Tween 20, 5% trehalose, pH 6.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.