1. 重组蛋白
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  4. PNCK 蛋白, Human (Sf9, GST)

PNCK 蛋白, Human (Sf9, GST)

目录号: HY-P701756
产品使用指南

PNCK 蛋白是一种钙/钙调蛋白依赖性蛋白激酶,是钙触发信号级联的关键组成部分。在体外,PNCK 表现出磷酸化 CREB1 和 SYN1/突触蛋白 I 的能力,强调其参与调节与这些底物相关的细胞过程。PNCK 蛋白, Human (Sf9, GST) 是重组的 PNCK 蛋白,由 Sf9 insect cells 表达,带GST标签。PNCK 蛋白, Human (Sf9, GST) 全长 342 个氨基酸。

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描述

The PNCK protein is a calcium/calmodulin-dependent protein kinase and a key component of the calcium-triggered signaling cascade. In vitro, PNCK exhibits the ability to phosphorylate CREB1 and SYN1/synapsin I, emphasizing its involvement in regulating cellular processes associated with these substrates. PNCK Protein, Human (Sf9, GST) is the recombinant human-derived PNCK protein, expressed by Sf9 insect cells , with GST tagged. The total length of PNCK Protein, Human (Sf9, GST) is 342 a.a., .

研究背景

PNCK 蛋白被鉴定为钙/钙调蛋白依赖性蛋白激酶,表明其参与了钙触发的信号级联反应。体外研究表明,PNCK 能够磷酸化 CREB1 和 SYN1(突触蛋白 I),表明其在调节与突触功能和基因表达相关的细胞过程中发挥作用。此外,PNCK 磷酸化并激活 CAMK1,表明其参与其他钙/钙调蛋白依赖性激酶的调节。这些发现强调了 PNCK 在细胞信号传导途径中的潜在重要性,特别是与钙介导事件相关的信号传导途径,并强调了其在参与突触功能和基因调控的关键蛋白的磷酸化和激活中的作用。需要进一步的研究来阐明 PNCK 介导的磷酸化事件的具体生物学背景和生理后果。

种属

Human

表达系统

Sf9 insect cells

标签

GST

蛋白编号

Q6P2M8-1 (L2-W343)

基因 ID

139728

同用名
PNCK; Calcium/calmodulin-dependent protein kinase type 1B; CaM kinase I beta; CaM kinase IB; CaM-KI beta; CaMKI-beta; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PNCK Protein, Human (Sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PNCK Protein, Human (Sf9, GST)
目录号:
HY-P701756
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