PSAT1 蛋白, Human (His)

The PSAT1 protein plays a central role in cellular metabolism by catalyzing the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. These enzymatic activities are pivotal steps in the biosynthetic pathway for the essential amino acids serine and threonine. PSAT1's ability to interconvert these metabolites contributes to the regulation of serine and threonine levels, critical for various cellular processes, including protein synthesis, nucleotide biosynthesis, and redox homeostasis. This underscores the integral role of PSAT1 in maintaining the balance of these amino acids, thereby influencing fundamental aspects of cellular metabolism and function. PSAT1 Protein, Human (His) is the recombinant human-derived PSAT1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of PSAT1 Protein, Human (His) is 370 a.a., with molecular weight of ~40 kDa.

PSAT1 蛋白通过催化 3-磷酸羟基丙酮酸可逆转化为磷酸丝氨酸以及 3-羟基-2-氧代-4-膦酰氧基丁酸可逆转化为磷酸羟基苏氨酸，在细胞代谢中发挥核心作用。这些酶活性是必需氨基酸丝氨酸和苏氨酸生物合成途径中的关键步骤。 PSAT1 相互转化这些代谢物的能力有助于调节丝氨酸和苏氨酸水平，这对于各种细胞过程至关重要，包括蛋白质合成、核苷酸生物合成和氧化还原稳态。这强调了 PSAT1 在维持这些氨基酸平衡中的不可或缺的作用，从而影响细胞代谢和功能的基本方面。

Measured by its ability to produce 3-phosphooxypyruvate. The specific activity is >130 pmol/min/μg.

Human

E. coli

C-6*His

Q9Y617-1 (M1-L370)

29968  [NCBI]

MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL

Approximately 40 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.