RNF5 蛋白, Human (Cell-Free, His)

The RNF5 protein is a membrane-bound E3 ubiquitin protein ligase that is critical for ubiquitination of target proteins and can cooperate with UBE2D1/UBCH5A and UBE2D2/UBC4 to achieve ubiquitination over a broad substrate range. Notably, it mediates PXN/paxillin ubiquitination, affecting cell motility and cell positioning. RNF5 Protein, Human (Cell-Free, His) is the recombinant human-derived RNF5 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of RNF5 Protein, Human (Cell-Free, His) is 179 a.a., with molecular weight of27 kDa&54 kDa& 108 kDa.

RNF5 蛋白是一种膜结合 E3 泛素蛋白连接酶，在靶蛋白泛素化中发挥着关键作用，表现出多种细胞功能。RNF5 与 E2 泛素结合酶（例如 UBE2D1/UBCH5A 和 UBE2D2/UBC4）合作，利用广泛的底物范围协调泛素化过程。值得注意的是，它介导 PXN/桩蛋白的泛素化，影响细胞运动和 PXN/桩蛋白的细胞定位。此外，RNF5 催化 III 型沙门氏菌分泌蛋白 sopA 和 JKAMP 的泛素化，调节其功能。JKAMP 的“Lys-63”连接的多聚泛素化调节其与蛋白酶体和 ERAD 成分的关联，而 STING1 在“Lys-150”处的“Lys-48”连接的多聚泛素化会导致蛋白酶体降解，影响抗病毒反应。此外，RNF5 催化 ATG4B 的泛素化和随后的降解，提供抑制自噬的机制。这些不同的活动凸显了 RNF5 在细胞过程中的多功能调节作用。

Human

E. coli Cell-free

N-10*His

Q99942 (A2-I180)

6048

AAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGTGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI

27 kDa&54 kDa&108 kDa. It is speculated that the protein forms a multimer structure.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.