SAG 蛋白, Human (P.pastoris, His)

SAG proteins play a crucial role in phototransduction, regulating signal transduction by binding to light-activated and phosphorylated rhodopsin (RHO). It terminates RHO signaling by competitively interacting with G proteins at the same binding site on RHO. SAG Protein, Human (P.pastoris, His) is the recombinant human-derived SAG protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of SAG Protein, Human (P.pastoris, His) is 405 a.a., with molecular weight of ~47.1 kDa.

SAG 蛋白通过与光激活和磷酸化视紫红质 (RHO) 结合，从而通过 G 蛋白终止 RHO 信号传导，从而在光转导中发挥至关重要的调节作用。通过与 RHO 上相同结合位点的 G 蛋白竞争性相互作用，SAG 以与类似蛋白一致的方式有效调节信号转导。此外，研究结果表明，SAG 可能有助于预防视网膜感光细胞的光依赖性变性。在结构上，SAG 可以单体、同二聚体或同四聚体的形式存在，突出了其多功能的寡聚状态。此外，SAG 与 RHO 发生特异性相互作用，特异性结合磷酸化的 C 末端，阐明了其在感光细胞分子动力学中的复杂参与。

Human

P. pastoris

N-6*His

P10523 (M1-E405)

6295  [NCBI]

MAASGKTSKSEPNHVIFKKISRDKSVTIYLGNRDYIDHVSQVQPVDGVVLVDPDLVKGKKVYVTLTCAFRYGQEDIDVIGLTFRRDLYFSRVQVYPPVGAASTPTKLQESLLKKLGSNTYPFLLTFPDYLPCSVMLQPAPQDSGKSCGVDFEVKAFATDSTDAEEDKIPKKSSVRLLIRKVQHAPLEMGPQPRAEAAWQFFMSDKPLHLAVSLNKEIYFHGEPIPVTVTVTNNTEKTVKKIKAFVEQVANVVLYSSDYYVKPVAMEEAQEKVPPNSTLTKTLTLLPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDRTVLGILVSYQIKVKLTVSGFLGELTSSEVATEVPFRLMHPQPEDPAKESYQDANLVFEEFARHNLKDAGEAEEGKRDKNDVDE

Approximately 47.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.