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  3. Semaphorin-3A/SEMA3A 蛋白, Rat (His)

Semaphorin-3A/SEMA3A 蛋白, Rat (His)

目录号: HY-P71564
COA 产品使用指南

Semaphorin-3A/SEMA3A 蛋白通过创建指导特定神经束形成的分子边界,在指导轴突生长方面发挥着潜在的关键作用。它与神经毡蛋白的结合涉及嗅觉系统发育和青春期的神经元调节。SEMA3A 与 PLXND1 的相互作用强调了其在轴突引导和神经发育中的重要性。Semaphorin-3A/SEMA3A 蛋白, Rat (His) 是重组的 Semaphorin-3A/SEMA3A 蛋白,由 E. coli 表达,带有 N-6*His 标签。Semaphorin-3A/SEMA3A 蛋白, Rat (His) 全长 752 个氨基酸,分子量约为 ~90.6 kDa。

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描述

Semaphorin-3A/SEMA3A protein plays a potentially crucial role in directing axon growth by creating molecular boundaries that guide the formation of specific nerve tracts. Its binding to neuropilin is implicated in olfactory system development and neuronal regulation of puberty. SEMA3A's interaction with PLXND1 underscores its significance in axon guidance and neural development. Semaphorin-3A/SEMA3A Protein, Rat (His) is the recombinant rat-derived Semaphorin-3A/SEMA3A protein, expressed by E. coli , with N-6*His labeled tag. The total length of Semaphorin-3A/SEMA3A Protein, Rat (His) is 752 a.a., with molecular weight of ~90.6 kDa.

研究背景

Semaphorin-3A/SEMA3A 蛋白通过创建为特定神经束的形成提供指令的分子边界,对于引导轴突向其预期目标生长可能至关重要。它与神经毡蛋白结合,参与嗅觉系统的发育和青春期的神经元调节。此外,SEMA3A 与 PLXND1 相互作用,进一步强调了其在轴突引导和神经发育中的重要性。

种属

Rat

表达系统

E. coli

标签

N-6*His

蛋白编号

Q63548 (21N-772V)

基因 ID

29751  [NCBI]

蛋白结构
N-term
6*His
SEMA3A (21N-772V)
Accession # Q63548
C-term
同用名
Sema3a; Semaphorin-3A; Semaphorin III; Sema III
氨基酸序列

NYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRPIMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEVLLEEMTVFREPTTISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRNGDPLTHCSDLQHHDNHHGHSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRVGDHIIRTEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSSSMTPSQKVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPRSV

分子量

Approximately 90.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Semaphorin-3A/SEMA3A Protein, Rat (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Semaphorin-3A/SEMA3A Protein, Rat (His)
目录号:
HY-P71564
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