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  3. SP-17 蛋白, Human (His)

SP-17 蛋白, Human (His)

目录号: HY-P701069
产品使用指南

SP-17 蛋白是一种重要的精子表面结合蛋白,可促进精子高亲和力附着于透明带,表明其在受精中的关键作用。它参与透明带和碳水化合物的结合,强调了它在受精过程中的重要性。SP-17 蛋白, Human (His) 是重组的 SP-17 蛋白,由 E. coli 表达,带有 N-His 标签。SP-17 蛋白, Human (His) 全长 151 个氨基酸,分子量为 20-28 kDa。

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描述

The SP-17 protein is an important sperm surface-binding protein that promotes high-affinity sperm attachment to the zona pellucida, indicating its critical role in fertilization. It is involved in the binding of the zona pellucida and carbohydrates, emphasizing its importance in the fertilization process. SP-17 Protein, Human (His) is the recombinant human-derived SP-17 protein, expressed by E. coli , with N-His labeled tag. The total length of SP-17 Protein, Human (His) is 151 a.a., with molecular weight of 20-28 kDa.

研究背景

SP-17 蛋白是重要的精子表面结合蛋白,促进精子与透明带的高亲和力附着。它的作用表明参与透明带和碳水化合物的结合,强调了它在受精过程中的重要性。该蛋白质以同二聚体形式存在,可能与 ROPN1 相互作用,从而促成复杂的分子相互作用,这对受精过程中精卵成功结合至关重要。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

Q15506 (M1-K151)

基因 ID
蛋白结构
N-term
His
SP-17 (M1-K151)
Accession # Q15506
C-term
同用名
Sperm surface protein Sp17; CT22; Sp17-1; Sperm protein 17; SPA17;
分子量

20-28 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SP-17 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HY-P701069
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