1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. TLK1
  5. TLK1 蛋白, Human (sf9, His-GST)

TLK1 蛋白, Human (sf9, His-GST)

目录号: HY-P76676
产品使用指南

TLK1 蛋白是细胞过程的一个组成部分,在 S 期响应 DNA 双链断裂,经历快速、短暂的磷酸化诱导的抑制。这种 ATM 通路依赖的检查点机制在染色质组装中发挥着至关重要的作用。TLK1 蛋白, Human (sf9, His-GST) 是重组的 TLK1 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。TLK1 蛋白, Human (sf9, His-GST) 全长 766 个氨基酸,分子量约为 ~125 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The TLK1 protein is an integral part of cellular processes and undergoes rapid, transient phosphorylation-induced inhibition during S phase in response to DNA double-strand breaks. This ATM pathway-dependent checkpoint mechanism plays a crucial role in chromatin assembly. TLK1 Protein, Human (sf9, His-GST) is the recombinant human-derived TLK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of TLK1 Protein, Human (sf9, His-GST) is 766 a.a., with molecular weight of ~125 kDa.

研究背景

TLK1 是一种复杂参与细胞过程的蛋白质,在 S 期发生 DNA 双链断裂时,会通过磷酸化经历快速且短暂的抑制。这种调节机制依赖于检查点,涉及 ATM 通路,并且在协调与染色质组装相关的过程中发挥着至关重要的作用。值得注意的是,TLK1 的异构体 3 表现出磷酸化和增强 t-SNARE SNAP23 稳定性的能力,从而促进其与突触融合蛋白的组装。此外,该同种型通过促进 DNA 双链断裂的修复来发挥针对电离辐射的保护作用。在体外,TLK1 在“Ser-10”位点磷酸化组蛋白 H3,进一步强调其参与染色质动力学和细胞对 DNA 损伤的反应。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q9UKI8 (M1-Y766)

基因 ID
蛋白结构
N-term
His-GST
TLK1 (M1-Y766)
Accession # Q9UKI8
C-term
同用名
Serine/threonine-protein kinase tousled-like 1; PKU-beta; Tousled-like kinase 1; TLK1; KIAA0137
分子量

Approximately 125 kDa

纯度

Greater than 88% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TLK1 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
TLK1 Protein, Human (sf9, His-GST)
目录号:
HY-P76676
需求量: