2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. Wee1 蛋白, Human (Sf9)

Wee1 蛋白, Human (Sf9)

目录号: HY-P701726
产品使用指南

Wee1 蛋白是 G2 到 M 转变的关键负调节因子，通过磷酸化“Tyr-15”上的 CDK1 并使细胞周期蛋白 B1-CDK1 复合物失活来确保核保护。随着细胞进入 M 期，其在 G2 期的峰值活性降低，并进行动态调节，S 期和 G2 期磷酸化增加，并且可能由于降解而在 M/G1 相转变期间水平降低。Wee1 蛋白, Human (Sf9) 是重组的 Wee1 蛋白，由 Sf9 insect cells 表达，不带标签。Wee1 蛋白, Human (Sf9) 全长 285 个氨基酸。

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3.  试用装只面向终端客户。

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100 μg   询价  

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描述

Wee1 Protein acts as a crucial negative regulator of the G2 to M transition, ensuring nuclear protection by phosphorylating CDK1 on 'Tyr-15' and inactivating the cyclin B1-CDK1 complex. Its peak activity in G2 phase decreases as cells progress into M phase, with dynamic regulation, increased phosphorylation during S and G2 phases, and diminished levels during M/G1 phase transition potentially due to degradation. Wee1 Protein, Human (Sf9) is the recombinant human-derived Wee1 protein, expressed by Sf9 insect cells , with tag free. The total length of Wee1 Protein, Human (Sf9) is 285 a.a., .
研究背景

Wee1 蛋白作为 G2 到 M 转变的关键负调节因子，通过保护细胞核免受细胞质结合的细胞周期蛋白 B1 复合物 CDK1 的激活来防止进入有丝分裂。通过“Tyr-15”上 CDK1 的磷酸化，Wee1 特异性靶向并灭活细胞周期蛋白 B1-CDK1 复合物，其活性在 G2 期达到峰值，并在细胞进入 M 期时达到最低值。值得注意的是，Wee1 对细胞周期蛋白 B1-CDK1 的磷酸化仅发生在“Tyr-15”上，而单体 CDK1 保持未磷酸化。其活性经历动态调节，在S期和G2期增加，在M期减少，并伴有过度磷酸化。此外，Wee1 蛋白水平在 M/G1 相变期间发生相关降低，可能归因于其降解。
种属

Human
表达系统

Sf9 insect cells
标签

Tag Free
蛋白编号

P30291 (M291-K575)
基因 ID

7465
蛋白结构
N-term
Wee1 (M291-K575)
Accession # P30291
C-term
同用名
WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Wee1 Protein, Human (Sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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