Robotnikinin 

Robotnikinin is a small molecule capable of binding to and inhibiting the activity of Sonic Hedgehog (Shh) signaling up stream of Smo^[1][2].

Robotnikinin demonstrates ShhN-binding capacity at concentrations between 1.56 μM and 25 μM, with a K_D of 3.1 μM derived from kinetic data^[2].
Robotnikinin (50 µM) prevents the ShhN-induced LC3-II increase^[3].
Robotnikinin (5 μM) downregulates classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells, suggesting a link between Hh signaling and the NF-κB pathway in MM^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

454.95

C₂₅H₂₇ClN₂O₄

1132653-79-2

固体

White to off-white

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

• [1]. Manuel Hitzenberger, et al. The Binding Mode of the Sonic Hedgehog Inhibitor Robotnikinin, a Combined Docking and QM/MM MD Study. Front Chem  [Content Brief]

  [2]. Benjamin Z Stanton, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6.  [Content Brief]

  [3]. Ronald S Petralia, et al. Sonic hedgehog promotes autophagy in hippocampal neurons. Biol Open. 2013 Apr 8;2(5):499-504.  [Content Brief]

  [4]. Ke Cai, et al. Targeting the cross-talk between the hedgehog and NF-κB signaling pathways in multiple myeloma. Leuk Lymphoma. 2019 Mar;60(3):772-781.  [Content Brief]