β-Amyloid (1-42), human, HFIP-treated 

β-Amyloid (1-42), human, HFIP-treated is a β-Amyloid (1-42), human (HY-P1363A) treated with HFIP. β-Amyloid (1-42), human (Amyloid β-Peptide (1-42) (human)) is a 42-amino acid peptide that causes neurotoxicity, which is related to the pathogenesis of Alzheimer's disease. β-Amyloid (1-42), human specifically interacts with the promoters of genes like LRP1 and KAI1. β-Amyloid (1-42), human can form oligomers and fibrils in vitro, and the oligomeric form is more neurotoxic than the fibrillar form^{[1][2][3][4][5][6]}.

β-淀粉样蛋白聚集指南 (以下是我们推荐的方案。此方案仅提供指南，应根据您的具体需要进行修改)。
1. 将 β-Amyloid (1-42), human, HFIP-treated 溶解在无水 DMSO 中，浓度为 5 mM，然后涡旋稀释成合适的浓度和缓冲液 (无血清和酚红的培养基)。
2. 接下来，将溶液在 4-8°C 下 48 小时。然后将样品在 4-8°C 下以 14000g 离心 10 分钟；可溶性低聚物在上清液中。将上清液稀释 10 -200 倍用于实验。
方法因下游应用而异。
注意：
聚集形式在溶液中不稳定，建议立即使用。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

4514.04

C₂₀₃H₃₁₁N₅₅O₆₀S

107761-42-2

粘稠液体

Colorless to light yellow

Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Solntseva EI, et al. Impact of amyloid-β peptide (1-42) on voltage-gated ion currents in molluscan neurons. Bull Exp Biol Med. 2011 Oct;151(6):671-4.  [Content Brief]

  [2]. Barucker C, et al. Nuclear translocation uncovers the amyloid peptide Aβ42 as a regulator of gene transcription. J Biol Chem. 2014 Jul 18;289(29):20182-91.  [Content Brief]

  [3]. Stefania Sabella, et al. Capillary electrophoresis studies on the aggregation process of beta-amyloid 1-42 and 1-40 peptides. Electrophoresis. 2004 Oct;25(18-19):3186-94.  [Content Brief]

  [4]. Vosough F, et al. Characterization of Homogeneous and Heterogeneous Amyloid-β42 Oligomer Preparations with Biochemical Methods and Infrared Spectroscopy Reveals a Correlation between Infrared Spectrum and Oligomer Size. ACS Chem Neurosci. 2021 Feb 3;12(3):473-488.  [Content Brief]

  [5]. Stine WB Jr, et al. In vitro characterization of conditions for amyloid-beta peptide oligomerization and fibrillogenesis. J Biol Chem. 2003 Mar 28;278(13):11612-22.  [Content Brief]

  [6]. Roher AE, et al. beta-Amyloid-(1-42) is a major component of cerebrovascular amyloid deposits: implications for the pathology of Alzheimer disease. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10836-40.  [Content Brief]