2. Autophagy
  3. Autophagy
  4. Leonurine

Leonurine  (Synonyms: 益母草碱; SCM-198)

目录号: HY-N0741 纯度: 99.86%
COA 产品使用指南 技术支持

Leonurine 是从益母草中分离到的生物碱，具有抗氧化、抗炎活性。

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Leonurine

Leonurine Chemical Structure

CAS No. : 24697-74-3

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规格 价格 是否有货 数量
1 mg ￥135
In-stock
5 mg ￥259
In-stock
10 mg ￥388
In-stock
25 mg ￥700
In-stock
50 mg ￥1050
In-stock
100 mg ￥1575
In-stock
500 mg ￥3713
In-stock
1 g   询价  
5 g   询价  

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Customer Review

Other Forms of Leonurine:

Leonurine 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Leonurine is an alkaloid isolated from Leonurus artemisia, with anti-oxidative and anti-inflammatory.

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
H9c2 ED50
192 nM
Compound: 11c; SCM-198
Antioxidant activity in rat H9c2 cells assessed as protection against H2O2-induced hypoxic injury by measuring cell viability preincubated for 4 hrs followed by H2O2 stimulation for 1 hr by MTT assay relative to control
Antioxidant activity in rat H9c2 cells assessed as protection against H2O2-induced hypoxic injury by measuring cell viability preincubated for 4 hrs followed by H2O2 stimulation for 1 hr by MTT assay relative to control
[PMID: 32485530]
体外研究
(In Vitro)

Leonurine (0、5、10、20、40、80 μM) 处理 24 小时后，可减少脂质积聚和细胞胆固醇含量，包括总胆固醇 (TC)、游离胆固醇 (FC) 和胆固醇酯 (CE)，并增加载脂蛋白 A-I 或高密度脂蛋白 (HDL) 介导的胆固醇外流。Leonurine 还能显著且剂量依赖性地增加人 THP-1 巨噬细胞中 ABCA1 和 ABCG1 的 mRNA 和蛋白质水平表达，此作用与 PPARγ 有关[1]。
Leonurine 对用棕榈酸 (PA) 或游离脂肪酸 (FFA) 孵育 24 小时的 HepG2 和 HL-7702 细胞活力有保护作用。Leonurine (125、250、500 μM) 通过激活 AMPK/SREBP1 通路促进 HepG2 和 HL-7702 细胞脂质蓄积[2]。
Leonurine (5、10、20 μM) 以剂量依赖性方式抑制 IL-1β 诱导的人软骨细胞中 iNOS、COX-2、PGE2、NO、TNF-α 和 IL-6 的表达，抑制人 OA 软骨细胞中的 ECM 降解，并阻断 IL-1β 诱导的 PI3K 和 Akt 磷酸化[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Leonurine 相关抗体:
体内研究
(In Vivo)

Leonurine (10 mg/kg/d，口服) 显著增加小鼠血清中 PPARγ、LXRα、ABCA1 和 ABCG1 的表达，并降低甘油三酸酯 (TG) 和总胆固醇 (TC) 水平[1]。
Leonurine (50、100、200 mg/kg) 通过激活 AMPK/SREBP1 通路改善细胞内脂质蓄积，改善小鼠生化指标，降低肝脏脂质过氧化物，并提高抗氧化水平[2]。
Leonurine (20 mg/kg，口服) 可改善小鼠DMM模型中的骨关节炎发展[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

311.33

Formula

C14H21N3O5
CAS 号
性状

固体

颜色

White to off-white

中文名称

益母草碱
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 30.3 mg/mL (97.32 mM; ultrasonic and adjust pH to 3 with 1 M HCl; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2120 mL 16.0601 mL 32.1203 mL
5 mM 0.6424 mL 3.2120 mL 6.4241 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.86%

COA (281 KB) HNMR (144 KB) LCMS (90 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[2]

MTT assay is performed to study the cytotoxic effects of Leonurinein HepG2 and HL-7702 cells. Briefly, HepG2 and HL-7702 cells are seeded for 24 h at the density of 3 × 104 cells/well in 96-well plates. After 24 h incubation, cells are treated with different concentrations of Leonurine (0-1000 μM) and the control group is treated with only DMEM for 24 h at 37°C in 5% CO2 incubator. Then, these cells are treated with MTT solution (5 mg/mL) for further 4 h. After 4 h incubation, DMEM containing MTT solution is discarded. Cells are then dissolved by adding DMSO (200 μL) to each well and the solutions are mixed thoroughly for 5 min. Finally, the absorbance is determined at 570 nm with a microplate reader[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Mice[1]
ApoE-/- mice (male, eight-week old) are fed a chow diet for 2 weeks, apoE-/- mice are randomly divided into several groups (n=15/group). Mice in the Leonurine group are intragastrically administered with Leonurine (10 mg/kg/d) every day and continued for 8 weeks. The control group is fed with an equal volume of PBS. At week 16, mice are euthanized, followed by collecting the blood and tissue samples for further analyses[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Leonurine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2120 mL 16.0601 mL 32.1203 mL 80.3006 mL
5 mM 0.6424 mL 3.2120 mL 6.4241 mL 16.0601 mL
10 mM 0.3212 mL 1.6060 mL 3.2120 mL 8.0301 mL
15 mM 0.2141 mL 1.0707 mL 2.1414 mL 5.3534 mL
20 mM 0.1606 mL 0.8030 mL 1.6060 mL 4.0150 mL
25 mM 0.1285 mL 0.6424 mL 1.2848 mL 3.2120 mL
30 mM 0.1071 mL 0.5353 mL 1.0707 mL 2.6767 mL
40 mM 0.0803 mL 0.4015 mL 0.8030 mL 2.0075 mL
50 mM 0.0642 mL 0.3212 mL 0.6424 mL 1.6060 mL
60 mM 0.0535 mL 0.2677 mL 0.5353 mL 1.3383 mL
80 mM 0.0402 mL 0.2008 mL 0.4015 mL 1.0038 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Leonurine24697-74-3SCM-198益母草碱SCM198SCM 198AutophagyInhibitorinhibitorinhibit

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