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  2. Dynamin Mitophagy Autophagy Apoptosis
  3. Mdivi-1

Mdivi-1  (Synonyms: Mitochondrial division inhibitor 1)

目录号: HY-15886 纯度: 99.73%
COA 产品使用指南

Mdivi-1 是选择性的发动蛋白相关蛋白 1 (Drp1) 抑制剂。Mdivi-1 是一种有效的线粒体分裂/线粒体自噬 (mitophagy) 抑制剂。

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Mdivi-1 Chemical Structure

Mdivi-1 Chemical Structure

CAS No. : 338967-87-6

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥500
In-stock
5 mg ¥400
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10 mg ¥642
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50 mg ¥2188
In-stock
100 mg ¥3063
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200 mg   询价  
500 mg   询价  

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 125 篇科研文献

WB
IHC

    Mdivi-1 purchased from MCE. Usage Cited in: Vet Microbiol. 2023 May.

    S.aureus-induced reduction of mitochondrial proteins is blocked by Mdivi-1 (20 μM; 2 h) in BMECs.

    Mdivi-1 purchased from MCE. Usage Cited in: Theranostics. 2019 Feb 28;9(6):1698-1713.  [Abstract]

    Western blotting of SDs and markers of podocyte dedifferentiation with the treatment of PA , BBR + PA and Mdivi1+PA.

    Mdivi-1 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.  [Abstract]

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 24 h. Mitochondrial colocalization with LC3 is detected by immunofluorescence.

    Mdivi-1 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.  [Abstract]

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 48 h. Apoptosis-related proteins expression level is detected by western blotting. β-actin was used as a loading control.

    Mdivi-1 purchased from MCE. Usage Cited in: Brain Res. 2019 May 15;1711:68-76.  [Abstract]

    Levels of CytC in cytosol (Cyto-CytC), CytC in mitochondria (Mito- CytC) and cleaved caspase-3 in hippocampus are determined by western blot with the treatment of sham, HIR, MDV or FK506.

    Mdivi-1 purchased from MCE. Usage Cited in: J Pineal Res. 2018 Sep;65(2):e12491.  [Abstract]

    Inhibition of mitochondrial fission with mdivi-1 or Drp1 siRNA suppresses hyperglycemia-induced oxidative stress. Protein expression of Nox4.

    Mdivi-1 purchased from MCE. Usage Cited in: Transl Res. 2018 Apr;194:68-78.  [Abstract]

    Representative western blot analysis of pDrp1-616 and total Drp1 protein expression in the hippocampus (left). Tubulin is used as the loading control. Protein expressions are quantified by western blot band intensity (right).

    Mdivi-1 purchased from MCE. Usage Cited in: Free Radic Biol Med. 2017 Nov;112:336-349.  [Abstract]

    Mdivi-1 administration reduces NF-κB-dependent inflammation. A. Western blot assay and quantitative analysis of Cyto-p65 (A) and Nec-p65 (B) expression. ß-actin and H3 are used as internal controls of cytosolic and nuclear subfractions, respectively.

    Mdivi-1 purchased from MCE. Usage Cited in: Exp Neurol. 2017 Sep;295:116-124.  [Abstract]

    Mdivi-1 treatment inhibits the expression of DRP1, alleviates ROS and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of DRP1.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor. Mdivi-1 is a mitochondrial division/mitophagy inhibitor.

    体外研究
    (In Vitro)

    Mdivi-1 以剂量依赖性方式抑制 Dnm1 GTPase 活性,估计 EC50 为 1-10 μM。Mdivi-1 增加 GTP 的表观 K0.5,降低 GTP 水解的表观 Vmax,并导致在 Dnm1 GTP 水解中观察到的 GTP 希尔系数增加反应[1]。用 Mdivi-1 处理的细胞在细胞凋亡诱导后显示细胞色素 c 释放减少,磷脂酰丝氨酸暴露率降低,这与细胞凋亡的抑制以及之前使用其他策略损害 DRP1 活性的研究一致[2]。Mdivi-1 导致缺血性视网膜发生凋亡性细胞死亡[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    线粒体分裂 DRP,Dnm1,是体内 mdivi-1 的靶标。Mdivi-1 在与其对体内线粒体分裂的影响相似的浓度范围内定量阻断 GMPPCP 依赖性 Dnm1 自组装[1]。Mdivi-1 (50 mg/kg,腹腔注射) 显著降低正常小鼠视网膜中的 GFAP 蛋白表达[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    353.22

    Formula

    C15H10Cl2N2O2S

    CAS 号
    性状

    固体

    颜色

    White to khaki

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 160 mg/mL (452.98 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.8311 mL 14.1555 mL 28.3110 mL
    5 mM 0.5662 mL 2.8311 mL 5.6622 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 4 mg/mL (11.32 mM); 澄清溶液

      此方案可获得 ≥ 4 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 40.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。

    该产品建议参考如下动物实验溶解方案,可直接配制工作。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: 0.5% CMC-Na/saline water

      Solubility: 2.5 mg/mL (7.08 mM); 悬浊液; 超声助溶

    • 方案 二

      请依序添加每种溶剂: 17% Polyethylene glycol 12-hydroxystearate in Saline

      Solubility: 10 mg/mL (28.31 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.73%

    参考文献
    Kinase Assay
    [1]

    All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8311 mL 14.1555 mL 28.3110 mL 70.7774 mL
    5 mM 0.5662 mL 2.8311 mL 5.6622 mL 14.1555 mL
    10 mM 0.2831 mL 1.4155 mL 2.8311 mL 7.0777 mL
    15 mM 0.1887 mL 0.9437 mL 1.8874 mL 4.7185 mL
    20 mM 0.1416 mL 0.7078 mL 1.4155 mL 3.5389 mL
    25 mM 0.1132 mL 0.5662 mL 1.1324 mL 2.8311 mL
    30 mM 0.0944 mL 0.4718 mL 0.9437 mL 2.3592 mL
    40 mM 0.0708 mL 0.3539 mL 0.7078 mL 1.7694 mL
    50 mM 0.0566 mL 0.2831 mL 0.5662 mL 1.4155 mL
    60 mM 0.0472 mL 0.2359 mL 0.4718 mL 1.1796 mL
    80 mM 0.0354 mL 0.1769 mL 0.3539 mL 0.8847 mL
    100 mM 0.0283 mL 0.1416 mL 0.2831 mL 0.7078 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    目录号:
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