2. Immunology/Inflammation
  3. Tim3
  4. Anti-Mouse TIM-3 Antibody (B8.2C12)

Anti-Mouse TIM-3 Antibody (B8.2C12) 

目录号: HY-P990004 纯度: 99.48%
COA 技术支持

Anti-Mouse TIM-3 Antibody (B8.2C12) 是一种抗小鼠 TIM-3 IgG1 单克隆抗体。Anti-Mouse TIM-3 Antibody (B8.2C12) 能阻断 Tim-3 与 Phosphatidylserine (PtdSer) 和 CEACAM1 的结合但不干扰与 Galectin-9 的结合。Anti-Mouse TIM-3 Antibody (B8.2C12) 可以抑制肿瘤生长并激活肿瘤浸润的 CD8+ T 细胞。Anti-Mouse TIM-3 Antibody (B8.2C12) 可用于研究癌症如乳腺癌和结肠癌以及实验性自身免疫性脑脊髓炎 (EAE) 模型的构建。

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Anti-Mouse TIM-3 Antibody (B8.2C12)

Anti-Mouse TIM-3 Antibody (B8.2C12) Chemical Structure

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Anti-Mouse TIM-3 Antibody (B8.2C12) 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Anti-Mouse TIM-3 Antibody (B8.2C12) is an anti-mouse TIM-3 IgG1 monoclonal antibody. Anti-Mouse TIM-3 Antibody (B8.2C12) can block the binding of Tim-3 with Phosphatidylserine (PtdSer) and CEACAM1 without interfering with the binding to Galectin-9. Anti-Mouse TIM-3 Antibody (B8.2C12) can inhibit tumor growth and activate tumor infiltrating CD8+ T cells. Anti-Mouse TIM-3 Antibody (B8.2C12) can be used for studying cancer such as breast cancer and colon cancer and constructing experimental autoimmune encephalomyelitis (EAE) models[1][2][3].

同型

Rat IgG1 kappa
推荐同型对照抗体
反应种属

Mouse
IC50 & Target

TIM-3
体外研究
(In Vitro)

Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL, 7 天) 可增加 C57/BL6 小鼠脾脏 CD4+CD25- T 细胞中 Th17 细胞的分化和 IL-17A 的产生[2]。
Anti-Mouse TIM-3 Antibody (B8.2C12) (4 μg/mL, 3 天) 促进实验性自身免疫性脑脊髓炎 (EAE) 小鼠脾脏中的 CD4+ T 细胞凋亡，但 CD8+ T 细胞没有显著影响[2]。
Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL) 可以阻断 Balb/c 小鼠胸腺细胞中 TIM-3 和 PtdSer 的结合，以及 Jurkat T 细胞中 TIM-3 与 CEACAM1 的结合[3]。
Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL) 不会阻断 TIM-3 与小鼠或人 Galectin-9 的结合[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse TIM-3 Antibody (B8.2C12) 相关抗体:

Apoptosis Analysis[2]

Cell Line: CD4+ T cells and CD8+ T cells
Concentration: 4 μg/mL
Incubation Time: 3 days
Result: Reduced the proportion of CD4+ T cells.
Had no significant effect on the proportion of CD8+ T cells.
体内研究
(In Vivo)

Anti-Mouse TIM-3 Antibody (B8.2C12) (100 μg，腹腔注射，在第 7、12 和 17 天) 与依托泊苷 (ETO) (HY-13629) 联合使用，可显著抑制携带 4T1 肿瘤的雌性 BALB/c 小鼠肿瘤生长并增加肿瘤内活化的CD8+ T 细胞[1]。
Anti-Mouse TIM-3 Antibody (B8.2C12) (300 μg，静脉注射，从免疫后第 8 天开始，持续 5 天) 成功构建了 C57/BL6 小鼠的实验性自身免疫性脑脊髓炎 (EAE) 模型[2]。
Anti-Mouse TIM-3 Antibody (B8.2C12) (200 μg，腹腔注射，每 3 天一次，共 2 次) 可能通过阻断配体结合或下调携带 CT26 肿瘤的野生型 Balb/c 小鼠的 TIM-3 来发挥抗肿瘤作用[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5×104 4T1 cells injected female BALB/c mice (6 weeks)[1]
Dosage: 100 μg, combined with ETO (50 mg/kg, i.v., on day 6 and 18)
Administration: Intraperitoneal injection (i.p.), on day 7, 12, and 17
Result: Significantly inhibited tumor volume.
Significantly reduced cancer stem cell (CSC) frequency.
Downregulated the level of STT3 in tumor cells.
Significantly increased CD8+ IFN-γ+ T cells.
Animal Model: C57/BL6 mice (7-8 weeks)[2]
Dosage: 300 μg, combined with MOG35-55 (HY-P3719)
Administration: Intravenous injection (i.v.), from day 8 post-immunization for 5 days
Result: Increased the proportion of CD4+ T cells and Th17 cells in the spinal cord.
Aggravated the symptoms of EAE.
Animal Model: 1×106 CT26 cells injected wild type Balb/c mice[3]
Dosage: 200 μg
Administration: Intraperitoneal injection (i.p.), once every 3 days for a total of 2 shots
Result: Did not alter the frequency of Treg cells within the tumor.
Might downregulate Tim-3 and exert anti-tumor effects.
基因 ID

171285  [NCBI]

Accession

Q8VIM0-1
应用

ELISA, FACS, Functional assay, Research in vivo
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
分子量

150 kDa

性状

液体

颜色

Colorless to light yellow

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Anti-Mouse TIM-3 Antibody (B8.2C12) 相关分类

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse TIM-3 Antibody (B8.2C12)Tim3T-Cell Immunoglobulin and Mucin Containing Protein-3CD366TIM-3breast cancercolon cancer4T1CT26EAEInhibitorinhibitorinhibit

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