1. MCE Kits
  2. Molecular Biology
  3. PCR & qPCR
  4. Polymerase Chain Reaction (PCR)
  5. 2× High-Fidelity PCR Master Mix

2× High-Fidelity PCR Master Mix 

2× 高保真 PCR 预混液

Cat. No.: HY-K0533
Manual SDS

2× High-Fidelity PCR Master Mix 是一款新型高保真高效率的即用型 PCR 扩增预混液,适用于高难度长片段的 PCR 扩增实验。

2× High-Fidelity PCR Master Mix

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample (100 μL)   Apply now  
1 mL ¥400 In-stock
5 mL ¥1428 In-stock

* Please select Quantity before adding items.

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE 2× High-Fidelity PCR Master Mix is a new high-fidelity PCR amplification premix. The product contains a new type of modified high-fidelity DNA Polymerase with higher 5’→3’polymerase activity and higher 3’→5’exonuclease activity. The additional hot-start factor can be activated to improve specificity and stability of PCR amplification by minimizing the non-specific amplification and enzyme activity loss. The product also contains a unique Extension-Enhancement factor, making the extending speed and the amplification efficient significantly improved. It can effectively amplify the fragments up to 10 kb, and the amplification speed can reach 15 sec/kb.

MCE 2× High-Fidelity PCR Master Mix is a ready-to-use premix. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, and provides consistent reaction-to-reaction performance. This product has no DNA loading buffer, the DNA loading buffer should be added to the PCR product when run on the agarose gel. The PCR product is blunt-ended.

Storage

Store at -20°C for 2 years. 4°C for short-term storage (up to 3 months). Avoid repetitive freeze-thaw cycles while using.

Components
Components HY-K0533- 1 mL HY-K0533- 5 mL
2× High-Fidelity PCR Master Mix 1 mL 1 mL × 5
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
2× 高保真 PCR 预混液
目录号:
HY-K0533
需求量: