Lentivirus Packaging Kit 

慢病毒包装试剂盒

慢病毒载体是以 HIV-1 为基础的基因治疗载体，能使外源基因稳定整合至宿主细胞基因组中，具有感染谱广泛、有效感染分裂期和静止期细胞、长期稳定表达外源基因等优点，因此成为导入外源基因的有力工具，广泛应用于各种细胞系的基因过表达、RNA 干扰、microRNA 研究及活体动物实验等。

MCE 慢病毒包装试剂盒基于第二代慢病毒包装系统，同时可兼容第二代和第三代慢病毒包装质粒。经 MCE 慢病毒包装试剂盒包装的慢病毒具有感染效率高、感染谱广等特点，可实现外源基因在宿主细胞中的稳定持续表达。

-20°C，1 年；4°C，6 个月

避免反复冻融。

1. Avoid repeated freeze/thaw cycles of plasmids. It is recommended to aliquot plasmids according to experimental needs and store them at -20°C for long-term preservation to ensure plasmid purity and transfection efficiency.

2. When collecting crude viral supernatant, in addition to routine aliquoting, it is advisable to prepare several small-volume aliquots (20-100 μL). Store these together with the main viral stock at -80°C. Once the small aliquots are fully frozen, thaw one aliquot and perform a titer determination. This simulates the titer reduction caused by a single freeze/thaw cycle, providing a more realistic and representative viral titer value that better reflects the actual infection efficiency.

3. All lentivirus-related procedures—including transfection, viral harvest, infection, and waste disposal—must be conducted in a BSL-2 (biosafety level 2) biosafety cabinet to ensure safe handling and containment.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.