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  4. 3D Cell Culture
  5. Basement Membrane Matrix
  6. Basement Membrane Matrix for Organoid Culture

Basement Membrane Matrix for Organoid Culture 

类器官基底膜基质胶

Cat. No.: HY-K6007
Manual SDS

MCE 类器官基底膜基质胶主要成分是小鼠肿瘤中提取的天然基底膜基质，主要用于培养类器官。

Basement Membrane Matrix for Organoid Culture

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (1 mL)   Apply now  
5 mL ￥2968 In-stock
10 mL ￥5397 In-stock

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  • Description

  • Shipping

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages
MCE Basement Membrane Matrix for Organoid Culture is primarily composed of natural basement membrane matrix extracted from mouse tumors. This product is mainly used for organoid culture.
Shipping
Shipping with dry ice.
Storage

-20°C, 2 years.

Avoid repeated freezing and thawing.
Protocol

Thin Gel Method. For cell migration and invasion, primary cell expansion: Dilute with pre-cooled medium according to experimental needs after thawing. It is recommended that the concentration be no less than 1 mg/mL. Add 50 μL/cm2 of matrix to the surface of the cell culture plate and spread it evenly, taking care to avoid air bubbles. Place the plate at 37 ℃ and incubate for 0.5h, aspirate the supernatant to use.

Thin Coating Method. For primary cell expansion: Dilute with pre-cooled medium according to experimental needs after thawing. It is recommended that the concentration be no less than 0.1 mg/mL. Aspirate the appropriate amount of dilution to completely cover the surface of the cell culture plate, the recommended amount of coating is 0.01-0.02 mg/cm2. Place the plate at 37 ℃ and incubate for 1h, aspirate the supernatant to use.

Gel embedding Method. For organoid culture and tumor spheroid invasion: Thaw the product in advance and set aside. Prepare the desired cells and resuspend them with matrix, it is recommended that the proportion of matrix is >70%. Add 15-20 μL/cm2 to the surface of the cell culture plate, taking care to avoid air bubbles. Place the plate at 37 ℃ for 0.5h to form a gel that wraps the cells, and add the appropriate medium to the plate.

Components
Components HY-K6007-5 mL HY-K6007-10 mL
Basement Membrane Matrix for Organoid Culture 5 mL 5 mL ×2
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
类器官基底膜基质胶
目录号：
HY-K6007
需求量：
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