1. Academic Validation
  2. Contractile effect of tachykinins on rabbit small intestine

Contractile effect of tachykinins on rabbit small intestine

  • Acta Pharmacol Sin. 2011 Apr;32(4):487-94. doi: 10.1038/aps.2010.227.
Marta Sofía Valero 1 Diego Santos Fagundes Laura Grasa María Pilar Arruebo Miguel Ángel Plaza María Divina Murillo
Affiliations

Affiliation

  • 1 Departamento de Farmacología y Fisiología (Fisiología), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, Zaragoza, Spain.
Abstract

Aim: To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P (SP).

Methods: Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath.

Results: The agonists of tachykinin NK1 receptor (SP and [Sar9] SP), NK2 receptor (NKA and (β-Ala8)-NKA), and NK3 receptor (NKB and Senktide) all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060, NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atropine, verapamil, PKC Inhibitor staurosporine, and PLC inhibitor U73122.

Conclusion: Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore, SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.

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