2. Academic Validation
  3. The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants

The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants

  • Cell Host Microbe. 2018 Sep 12;24(3):379-391.e5. doi: 10.1016/j.chom.2018.08.007.
Meixiang Zhang 1,  Yi-Hsuan Chiang 2,  Tania Y Toruño 2,  DongHyuk Lee 2,  Miaomiao Ma 3,  Xiangxiu Liang 3,  Neeraj K Lal 4,  Mark Lemos 5,  Yi-Ju Lu 6,  Shisong Ma 4,  Jun Liu 2,  Brad Day 6,  Savithramma P Dinesh-Kumar 4,  Katayoon Dehesh 5,  Daolong Dou 7,  Jian-Min Zhou 3,  Gitta Coaker 8
Affiliations

Affiliations

  • 1 Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China; Department of Plant Pathology, University of California, Davis, Davis, CA 95616, USA.
  • 2 Department of Plant Pathology, University of California, Davis, Davis, CA 95616, USA.
  • 3 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • 4 Department of Plant Biology and the Genome Center, University of California, Davis, Davis, CA 95616, USA.
  • 5 Department of Plant Biology, University of California, Davis, Davis, CA 95616, USA; Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, USA.
  • 6 Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USA.
  • 7 Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
  • 8 Department of Plant Pathology, University of California, Davis, Davis, CA 95616, USA. Electronic address: glcoaker@ucdavis.edu.
PMID: 30212650 DOI: 10.1016/j.chom.2018.08.007
Abstract

Microbial patterns are recognized by cell-surface receptors to initiate pattern-triggered immunity (PTI) in Plants. Receptor-like cytoplasmic kinases (RLCKs), such as BIK1, and calcium-dependent protein kinases (CPKs) are engaged during PTI to activate the NADPH Oxidase RBOHD for Reactive Oxygen Species (ROS) production. It is unknown whether protein kinases besides CPKs and RLCKs participate in RBOHD regulation. We screened mutants in all ten Arabidopsis MAP4 kinases (MAP4Ks) and identified the conserved MAP4K SIK1 as a positive regulator of PTI. SIK1 mutants were compromised in their ability to elicit the ROS burst in response to microbial features and exhibited compromised PTI to Bacterial infection. SIK1 directly interacts with, phosphorylates, and stabilizes BIK1 in a kinase activity-dependent manner. Furthermore, SIK1 directly interacts with and phosphorylates RBOHD upon flagellin perception. Thus, SIK1 positively regulates immunity by stabilizing BIK1 and activating RBOHD to promote the extracellular ROS burst.

Keywords

BIK1; MAP4K; PTI; RBOHD; ROS; SIK1; autoimmunity; phosphorylation.

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