1. Academic Validation
  2. Transcriptomics and metabolomics reveal the induction of flavonoid biosynthesis pathway in the interaction of Stylosanthes-Colletotrichum gloeosporioides

Transcriptomics and metabolomics reveal the induction of flavonoid biosynthesis pathway in the interaction of Stylosanthes-Colletotrichum gloeosporioides

  • Genomics. 2021 Jul;113(4):2702-2716. doi: 10.1016/j.ygeno.2021.06.004.
Lingyan Jiang 1 Pengpeng Wu 1 Liyun Yang 1 Chun Liu 1 Pengfei Guo 1 Hui Wang 1 Shaocai Wang 1 Fupeng Xu 1 Qiwang Zhuang 1 Xinzhuo Tong 1 Pandao Liu 2 Lijuan Luo 3
Affiliations

Affiliations

  • 1 Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Hainan 570228, PR China.
  • 2 Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Hainan 570228, PR China.
  • 3 Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Hainan 570228, PR China. Electronic address: 990908@hainanu.edu.cn.
Abstract

Colletotrichum, a hemibiotrophic Fungal pathogen with a broad host range, causes a yield-limiting disease called anthracnose. Stylo (Stylosanthes) is a dominant pasture legume in tropics and subtropics, and anthracnose is one of its most destructive disease. Resistance mechanisms against anthracnose in stylo are poorly understood, thus hindering the development of resistant varieties. We performed time-resolved leaf transcriptomics, metabolomics and in vitro inhibition assay to investigate the defense responses against Colletotrichum gloeosporioides in stylo. Transcriptomics demonstrated that flavonoid biosynthetic genes were significantly induced during the Infection. Consistently, metabolomics also showed the increased accumulation of flavonoid compounds. In vitro assays showed that phloretin and naringenin inhibited the mycelial growth, and apigenin, daidzein, quercetin and kaempferol suppressed conidial germination of Colletotrichum strains. Together, our results suggest that stylo Plants cope with C. gloeosporioides by up-regulation of genes and compounds in flavonoid biosynthesis pathway, providing potential targets for resistance breeding.

Keywords

Colletotrichum; Defense responses; Flavonoid; Metabolomics; Stylosanthes; Transcriptomics.

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