2. Academic Validation
  3. The whitening effect of cuscutin responsible for traditional use of Bergenia purpurascens

The whitening effect of cuscutin responsible for traditional use of Bergenia purpurascens

  • J Ethnopharmacol. 2024 Feb 19:117933. doi: 10.1016/j.jep.2024.117933.
Yang-Yang Liu 1,  Yue Zhang 1,  Ling Jiang 1,  Qing-Yu Lu 1,  Rui-Han Ye 1,  Zheng-Yu Guo 2,  Yun-Li Zhao 3,  Xiao-Dong Luo 4
Affiliations

Affiliations

  • 1 Yunnan Characteristic Plant Extraction Laboratory, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Key Laboratory of Research and Development for Natural Products, School of Pharmacy, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, PR China.
  • 2 Yunnan Botanee Bio-technology Group Co., Ltd., Yunnan Yunke Characteristic Plant Extraction Laboratory Co., Ltd., Yunnan, 650106, PR China. Electronic address: zhongwei@winona.cn.
  • 3 Yunnan Characteristic Plant Extraction Laboratory, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Key Laboratory of Research and Development for Natural Products, School of Pharmacy, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, PR China. Electronic address: zhaoyunli@ynu.edu.cn.
  • 4 Yunnan Characteristic Plant Extraction Laboratory, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Key Laboratory of Research and Development for Natural Products, School of Pharmacy, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China. Electronic address: xdluo@ynu.edu.cn.
PMID: 38382653 DOI: 10.1016/j.jep.2024.117933
Abstract

Ethnopharmacological relevance: The roots and rhizomes of Bergenia purpurascens (Hook. f. et Thomson) Engl., was used as a Sunscreen to protect against ultraviolet rays in Tibet of China historically, but its skin whitening constituents and pharmacological effects of this plant remained unknown.

Aim of the study: To investigate the anti-melanogenesis effect of B. purpurascens in vitro and in vivo, and then explore the preliminary mechanism.

Materials and methods: An ultraviolet B (UVB)-induced skin injury model of mice was used to verify the ameliorative effect of B. purpurascens extract (BPE) on ultraviolet damage. Then, alpha-melanocyte stimulating hormone (α-MSH)-induced murine melanoma cell line (B16F10) melanin generation model was further adopted to approval the effects of BPE and its bioactive compound, cuscutin, in vitro. Moreover, α-MSH stimulated melanogenesis model in zebrafish was employed to confirm the anti-pigmentation effect of cuscutin. Then, proteins expressions associated with melanin production were observed using western blotting assay to explore preliminary mechanism.

Results: BPE inhibited UVB-induced mice injury and restored skin barrier function observably in vivo. BPE and cuscutin suppressed the overproduction of melanin in α-MSH induced B16F10 significantly, in which cuscutin exhibited better effect than well-known Whitening Agent α-arbutin at same 10 μg/mL concentration. Moreover, the pigmentation of zebrafish embryo was decreased by cuscutin. Finally, cuscutin showed significant downregulation of expressions of Tyrosinase (TYR) and Tyrosinase related protein-1 (TRP-1), TRP-2 and microphthalmia-associated transcription factor (MITF) in the melanogenic signaling pathway.

Conclusion: B. purpurascens extract and its major bioactive constituent, cuscutin, showed potent anti-melanogenesis and skin-whitening effect by targeting TYR and TRP-2 proteins for the first time, which supported its traditional use.

Keywords

Anti-melanogenesis; Bergenia purpurascens; Cuscutin; Whitening.

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