Methylene blue (purity≥70%)  (Synonyms: Basic Blue 9 (purity≥70%); CI-52015 (purity≥70%); Methylthioninium chloride (purity≥70%))

Methylene blue (purity≥70%) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue (purity≥70%) is a vasopressor and is often used as a dye in several medical procedures. Methylene blue (purity≥70%) through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue (purity≥70%) is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue (purity≥70%) is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation^[1][2][3].

1. 样品处理：
1.1 对于石蜡切片：常规脱蜡复水。
1.2 对于冰冻切片：蒸馏水 2 min。
1.3 对于培养细胞：用 4% 多聚甲醛固定。蒸馏水洗涤 2 min。换用新鲜的蒸馏水，再洗涤 2 min。
2. Methylene blue 染色
2.1 用 0.1% Methylene blue 染色，2 min。
2.2 用蒸馏水充分洗涤，进行观察和拍照。
2.3 染色结果：
组织或细胞：蓝色

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

319.86

C₁₆H₁₈ClN₃S

61-73-4

固体

Brown to reddish brown

亚甲基蓝 (纯度≥70%)

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

• [1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72.  [Content Brief]

  [2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25.  [Content Brief]

  [3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38.  [Content Brief]