AGER 蛋白, Human (HEK293, hFc)

AGER proteins are cell surface pattern recognition receptors that expertly sense endogenous stress signals utilizing an extensive library of ligands, including advanced glycation end products, S100 proteins, high mobility Group Box 1 proteins/HMGB1, starch Like protein β/APP oligomers, nucleic acids, phospholipids, and glycosaminoglycans. AGER Protein, Human (HEK293, hFc) is the recombinant human-derived AGER protein, expressed by HEK293 , with C-hFc labeled tag. The total length of AGER Protein, Human (HEK293, hFc) is 321 a.a., with molecular weight of approximately 76.18 kDa.

AGER 蛋白是一种细胞表面模式识别受体，能够利用广泛的配体库熟练地感知内源性应激信号，包括高级糖基化终产物、S100 蛋白、高迁移率组盒 1 蛋白/HMGB1、淀粉样蛋白 β/APP 寡聚物、核酸、磷脂和糖胺聚糖。高级糖基化终产物的积累，特别是在衰老和糖尿病中普遍存在，会引发各种疾病部位的炎症反应，包括糖尿病、血管并发症、神经退行性疾病和癌症。RAGE 在配体结合后，利用 TIRAP 和 MYD88 作为接头来转导信号，最终通过 NF-κ-B 激活诱导炎症细胞因子 IL6、IL8 和 TNFα。值得注意的相互作用包括 S100A12 触发的细胞激活、S100B 诱导的心肌梗塞后细胞凋亡以及促进皮质神经元中淀粉样蛋白-β 肽易位。AGER 还通过 RAGE/SRC/Caveolin-1 途径在内皮白蛋白与 HMGB1 的转胞吞作用中发挥作用，导致内皮细胞通透性过高，并通过 NLRP3 炎性小体介导细胞外囊泡中 HMGB1 的负载，导致肝细胞焦亡。此外，它还能促进细胞外低甲基化 DNA 的摄取，从而激活炎症反应。组成型同二聚体和寡聚体形式，以及与 S100 蛋白、APP、TIRAP 和 HMGB1 的相互作用，凸显了非动物源 AGER 蛋白在各种细胞过程和病理条件中的复杂参与。

1.Immobilized Human AGER at 20 μg/mL (100 μL/well) can bind Biotinylated Human HMGB1.The ED₅₀ for this effect is 30.85 ng/mL, corresponding to a specific activity is 3.24×10⁴ Unit/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized human S100A12 at 2 μg/mL (100 μl/well) can bind recombinant human AGER with a linear range of 0.032-20 μg/mL.

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  Immobilized Human AGER at 20 μg/mL (100 μL/well) can bind Biotinylated Human HMGB1.The ED₅₀ for this effect is 30.85ng/mL, corresponding to a specific activity is 3.24×10⁴ Unit/mg.

Human

HEK293

C-hFc

Q15109 (Q24-A344)

177  [NCBI]

QNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLALA

approximately 76.18 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.