1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. Alanyl-tRNA synthetase 蛋白, Human (sf9, His)

Alanyl-tRNA synthetase 蛋白, Human (sf9, His)

目录号: HY-P75517
产品使用指南

丙氨酰-tRNA 合成酶蛋白促进两步过程,用 ATP 激活丙氨酸形成 Ala-AMP,并将其转移到 tRNA (Ala) 的受体末端。此外,它还通过其编辑域纠正带错误电荷的 tRNA(Ala)。Alanyl-tRNA synthetase 蛋白, Human (sf9, His) 是重组的 丙氨酰-tRNA 合成酶蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。Alanyl-tRNA synthetase 蛋白, Human (sf9, His) 全长 968 个氨基酸,分子量约为 ~109.2 kDa。

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描述

The Alanyl-tRNA synthetase protein facilitates a two-step process, activating alanine with ATP to form Ala-AMP and transferring it to the acceptor end of tRNA(Ala). Additionally, it corrects incorrectly charged tRNA(Ala) through its editing domain. Alanyl-tRNA synthetase Protein, Human (sf9, His) is the recombinant human-derived Alanyl-tRNA synthetase protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Alanyl-tRNA synthetase Protein, Human (sf9, His) is 968 a.a., with molecular weight of ~109.2 kDa.

研究背景

丙氨酰-tRNA 合成酶是蛋白质合成中的关键角色,它通过细致的两步过程协调丙氨酸与 tRNA (Ala) 的附着。最初,丙氨酸被 ATP 激活,导致 Ala-AMP 的形成。随后,该活化的丙氨酸被努力转移至 tRNA(Ala) 的受体末端。除了其主要的氨酰化功能外,丙氨酰-tRNA 合成酶还表现出显着的编辑域,使其能够纠正带错误电荷的 tRNA(Ala)。这种双重功能强调了丙氨酰-tRNA 合成酶在维持翻译过程的准确性和保真度方面的关键作用,强调了其在细胞蛋白质合成中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

P49588 (M1-N968)

基因 ID

16  [NCBI]

蛋白结构
N-term
His
AARS1 (M1-N968)
Accession # P49588
C-term
同用名
Alanine--tRNA ligase, cytoplasmic; AlaRS; AARS1; AARS
分子量

Approximately 109.2 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Alanyl-tRNA synthetase Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Alanyl-tRNA synthetase Protein, Human (sf9, His)
目录号:
HY-P75517
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