alpha Actinin 4/ACTN4 蛋白, Human (HEK293, C-His)

Alpha Actinin 4, a spectrin gene superfamily member, binds actin in various cell types. It localizes to microfilament bundles, adherens-type junctions, and Z-discs, facilitating actin-membrane binding. This gene also encodes a nonmuscle isoform involved in metastasis. Mutations in this gene are linked to glomerulosclerosis. With broad expression in tissues like placenta (RPKM 105.3) and kidney (RPKM 104.8), Alpha Actinin 4 is crucial in cellular dynamics and disease mechanisms. alpha Actinin 4 (ACTN4) Protein,Human (HEK293,C-His) is the recombinant human-derived alpha Actinin 4, expressed by HEK293 , with C-10*His labeled tag. The total length of alpha Actinin 4 (ACTN4) Protein,Human (HEK293,C-His) is 911 a.a., with molecular weight of ~106-120 kDa.

Alpha Actinin 4 是血影蛋白基因超家族的成员，作为肌动蛋白结合蛋白在各种细胞类型中发挥着多方面的作用。非肌肉亚型沿着微丝束和粘附型连接分布，促进非肌肉细胞中肌动蛋白-膜的结合。相反，骨骼肌、心脏和平滑肌亚型定位于 Z 盘和类似的致密体，锚定肌原纤维肌动蛋白丝。在细胞质中，该基因编码非肌肉α肌动蛋白亚型，据信参与转移过程。值得注意的是，该基因的突变与局灶性和节段性肾小球硬化症有关，凸显了其在细胞结构和疾病病理学中的重要性。 Alpha Actinin 4 在胎盘 (RPKM 105.3)、肾脏 (RPKM 104.8) 和其他 25 种组织等组织中普遍表达，在细胞动力学和疾病相关机制中发挥着关键作用。

Human

HEK293

C-10*His

NP_004915 (M1-L911)

81

MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL

Approximately 106-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.