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  4. Alpha-Fetoprotein 蛋白, Canine (HEK293, His)

Alpha-Fetoprotein 蛋白, Canine (HEK293, His)

目录号: HY-P700651
产品使用指南

甲胎蛋白结合铜、镍和脂肪酸，对胆红素表现出特殊的亲和力，尽管效率低于血清白蛋白。其结构变化，包括二聚体和三聚体形式以及单体状态，有助于该蛋白质的多功能性。这种与不同配体分子相互作用的复杂性强调了甲胎蛋白在各种生理过程中的重要性。Alpha-Fetoprotein 蛋白, Canine (HEK293, His) 是重组的 犬甲胎蛋白，由 HEK293 表达，带有 C-His 标签。Alpha-Fetoprotein 蛋白, Canine (HEK293, His) 全长 591 个氨基酸，分子量为 68-70 kDa。

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描述

Alpha-Fetoprotein binds copper, nickel, and fatty acids, displaying particular affinity for bilirubin, albeit less efficiently than serum albumin. Its structural variations, including dimeric and trimeric forms, alongside the monomeric state, contribute to the protein's versatile functionality. This complexity in molecular interactions with diverse ligands underscores Alpha-Fetoprotein's significance in various physiological processes. Alpha-Fetoprotein Protein, Canine (HEK293, His) is the recombinant canine-derived Alpha-Fetoprotein protein, expressed by HEK293 , with C-His labeled tag. The total length of Alpha-Fetoprotein Protein, Canine (HEK293, His) is 591 a.a., with molecular weight of 68-70 kDa.
研究背景

甲胎蛋白表现出多种结合能力，包括结合铜、镍和脂肪酸的能力。值得注意的是，它表现出对胆红素的亲和力，尽管效率低于血清白蛋白。该蛋白质以多种形式存在，除了单体形式外，还鉴定出二聚体和三聚体结构。这些结构变化可能有助于甲胎蛋白的功能多样性，表明其与不同配体的分子相互作用存在复杂的相互作用，并强调其在各种生理过程中的重要性。
种属

Canine
表达系统

HEK293
标签

C-His
蛋白编号

Q8MJU5 (R19-V609)
基因 ID

403551  [NCBI]
蛋白结构
N-term
Alpha-Fetoprotein (R19-V609)
Accession # Q8MJU5
His
C-term
同用名
rHuAlpha-Fetoprotein, His; AFP; Alpha-Fetoprotein
分子量

68-70 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Alpha-Fetoprotein Protein, Canine (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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