Aminopeptidase A 蛋白, Human (sf9, His)

Aminopeptidase A protein has calcium-regulating activity and plays a key role in central hypertension by preferentially cleaving N-terminal acidic residues, including angiotensin II. The specificity of this enzyme highlights its involvement in the regulation of blood pressure-related peptides, suggesting potential implications for the physiological mechanisms of hypertension. Aminopeptidase A Protein, Human (sf9, His) is the recombinant human-derived Aminopeptidase A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Aminopeptidase A Protein, Human (sf9, His) is 917 a.a., with molecular weight of ~115.5 kDa.

氨基肽酶 A 蛋白在中枢性高血压的调节中发挥着关键作用，表明钙调节偏好从肽（包括血管紧张素 II）中裂解 N 末端酸性残基。这种酶活性凸显了它参与与血压调节相关的肽的调节，表明对高血压的生理机制有潜在影响。氨基肽酶 A 的钙依赖性特异性强调了其在生物活性肽加工中复杂的调节作用，强调了其在心血管功能中的重要性以及在高血压情况下的潜在相关性。

Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin (Glu-AMC) and the specific activity is >2000 pmol/min/μg.

Human

Sf9 insect cells

N-His

Q07075 (R41-G957)

2028  [NCBI]

RSCDSSGDGGPGTAPAPSHLPSSTASPSGPPAQDQDICPASEDESGQWKNFRLPDFVNPVHYDLHVKPLLEEDTYTGTVSISINLSAPTRYLWLHLRETRITRLPELKRPSGDQVQVRRCFEYKKQEYVVVEAEEELTPSSGDGLYLLTMEFAGWLNGSLVGFYRTTYTENGQVKSIVATDHEPTDARKSFPCFDEPNKKATYTISITHPKEYGALSNMPVAKEESVDDKWTRTTFEKSVPMSTYLVCFAVHQFDSVKRISNSGKPLTIYVQPEQKHTAEYAANITKSVFDYFEEYFAMNYSLPKLDKIAIPDFGTGAMENWGLITYRETNLLYDPKESASSNQQRVATVVAHELVHQWFGNIVTMDWWEDLWLNEGFASFFEFLGVNHAETDWQMRDQMLLEDVLPVQEDDSLMSSHPIIVTVTTPDEITSVFDGISYSKGSSILRMLEDWIKPENFQKGCQMYLEKYQFKNAKTSDFWAALEEASRLPVKEVMDTWTRQMGYPVLNVNGVKNITQKRFLLDPRANPSQPPSDLGYTWNIPVKWTEDNITSSVLFNRSEKEGITLNSSNPSGNAFLKINPDHIGFYRVNYEVATWDSIATALSLNHKTFSSADRASLIDDAFALARAQLLDYKVALNLTKYLKREENFLPWQRVISAVTYIISMFEDDKELYPMIEEYFQGQVKPIADSLGWNDAGDHVTKLLRSSVLGFACKMGDREALNNASSLFEQWLNGTVSLPVNLRLLVYRYGMQNSGNEISWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLKDTNLIKTQDVFTVIRYISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFNTELQLWQMESFFAKYPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG

Approximately 115.5 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM, Tris 500 mM NaCl, 10 % glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.